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微塑料暴露对小鼠学习记忆的影响及其作用机制

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目的 探讨微塑料(MPs)暴露对小鼠学习记忆的影响，观察MPs暴露对脑源性神经营养因子(BDNF)/酪氨酸激酶受体B(TrkB)/N-甲基-D-天冬氨酸受体2B亚基(NR2B)信号通路蛋白表达和神经发生的影响，探索MPs暴露对小鼠学习记忆影响的作用机制。方法 40只雄性C57BL/6小鼠随机分为对照组(Ctrl)和微塑料暴露组(MPs)，MPs组小鼠以微塑料200 μl[0。3mg/(kg·d)]连续30 d灌胃。Morris水迷宫实验检测小鼠学习记忆能力;Western blotting检测小鼠海马BDNF、TrkB和NR2B蛋白水平;免疫荧光染色观察小鼠海马区双皮质素(DCX)和神经元核抗原(NeuN)阳性细胞数量，评估海马神经发生。结果 与对照组小鼠相比，MPs暴露组小鼠学习记忆能力显著降低(P＜0。01)，海马BDNF、TrkB和NR2B表达也显著低于对照组(P＜0。05)。MPs组小鼠海马DCX和NeuN阳性细胞数量显著低于对照组(P＜0。01)。结论 MPs暴露引起的学习记忆损伤可能与抑制BDNF/TrkB/NR2B信号通路，减少海马神经发生有关。
Effects of microplastics exposure on learning and memory in mice and its mechanism
Objective To investigate the effect of microplastic(MPs)exposure on learning and memory in mice,and its mechanism by observing the protein expression of brain-derived neurotrophic factor(BDNF)/tyrosine receptor kinase B(TrkB)/N-methyl-D-aspartate receptor subtype 2B(NR2B)signaling pathway and neurogenesis.Methods Forty male C57BL/6 mice were randomly divided into control group(Ctrl)and microplastics exposure group(MPs).Mice in MPs group were treated with 0.3 mg/(kg·d)microplastics,administered by gavage at a volume of 200 μl for 30 consecutive days.Morris water maze test was used to evaluate the spatial learning and memory ability of mice.Western blotting was used to detect the protein levels of BDNF,TrkB and NR2B in hippocampus of mice.Immunofluorescent staining was used to observe the number of doublecortin(DCX)and neuronal nuclei antigen(NeuN)positive cells in the hippocampus of mice to evaluate hippocampal neurogenesis.Results Compared with the control group,the ability of learning and memory decreased significantly in MPs group mice(P＜0.01).The expression levels of BDNF,TrkB and NR2B in the hippocampus of MPs group mice were significantly lower than those of control group(P＜0.05).The number of DCX and NeuN positive cells in the hippocampus of MPs group was significantly lower than that of control group(P＜0.01).Conclusion MPs exposure induces learning and memory impairment which may be related to inhibiting BDNF/TrkB/NR2B signaling pathway and reducing hippocampal neurogenesis.

MicroplasticsLearningMemoryBrain-derived neurotrophic factorTyrosine receptor kinase BN-methyl-D-aspartate receptor subtype 2BNeurogenesisWestern blottingMouse

姜新泽、刘强、孙旭、侯姜姗、马瑞、程梅、吴玉龙

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滨州医学院基础医学院病原生物学系,山东烟台 264003

滨州医学院附属医院麻醉科,山东滨州 256699

滨州医学院护理学院,山东 烟台 264003

微塑料 学习 记忆 脑源性神经营养因子 酪氨酸激酶受体B N-甲基-D-天冬氨酸受体2B亚基 神经发生 免疫印迹法 小鼠

山东省自然科学基金烟台市科技计划项目滨州医学院"临床+X"科技创新项目

ZR2022MH1092022JCYJ027BY2021LCX13

2024

10.16098/j.issn.0529-1356.2024.05.004

解剖学报
中国解剖学会

解剖学报

CSTPCD
影响因子：0.462
ISSN：0529-1356
年,卷(期)：2024.55(5)