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多巴胺受体在小肠的分布与定位

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目的 探讨多巴胺受体D3～D5在不同物种小肠的分布和定位。方法 应用免疫组织化学和免疫印迹法，分别检测D3～D5在小鼠、大鼠和恒河猴小肠的分布及表达水平，通过免疫荧光双标技术观察小肠固有层(LP)免疫球蛋白A阳性浆细胞(IgA+PC)中D3～D5的表达。结果 D3和D5在小鼠、大鼠和恒河猴小肠的上皮层、LP、黏膜下神经丛(SMP)和肌间神经丛(MP)内广泛分布;D4在小鼠和恒河猴小肠的分布与D3和D5一致，但在大鼠小肠仅分布于上皮层和LP。小鼠和大鼠LP内的IgA+PC均表达D3和D5，不表达D4。结论 D3和D5在小鼠、大鼠和恒河猴小肠具有相似的分布和定位模式，D4则存在物种差异。多巴胺可能参与调节IgA+PC的功能。
Distribution and localization of dopamine receptor in small intestines
Objective To explore the distribution and localization of dopamine receptor D3-D5 in the small intestine of different species.Methods The distribution and expression of D3-D5 in the small intestine of mice,rats and rhesus monkeys were detected by immunohistochemistry and Western blotting.The expression of D3-D5 in immunoglobulin A positive plasma cells(IgA+PC)located in the lamina propria(LP)were detected by immunofluorescence double labeling.Results D3 and D5 were widely distributed in the epithelium,LP,submucosal plexus(SMP)and intermuscular plexus(MP)of the small intestine in mice,rats and rhesus monkeys.The distribution of D4 in the small intestinal of mice and rhesus monkeys were consistent with the result of D3 and D5.D4 was distributed only within the epithelium and LP of rat small intestine.D3 and D5 were expressed in the IgA+PC in the LP of mice and rats,whereas D4 was not.Conclusion The distribution and localization pattern of D3 and D5 are similar in the small intestine of mice,rats and rhesus monkeys,whereas those of D4 vary between different species.Dopamine may be involved in regulating the functions of IgA+PC.

Dopamine receptorSmall intestineImmunoglobulin A positive plasma cellWestern blottingImmunofluorescenceMouseRatRhesus monkey

陈钧钧、周立、苏田、王现伟、张海龙、王志勇

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新乡医学院人体解剖学与组织学胚胎学系,河南新乡 453003

河南省医用组织再生重点实验室,河南新乡 453003

河南大学基础医学院,河南开封 475000

多巴胺受体 小肠 免疫球蛋白A阳性浆细胞 免疫印迹法 免疫荧光 小鼠 大鼠 恒河猴

河南省科技发展计划项目新乡医学院博士科研启动基金河南省高等学校青年骨干教师培养计划

[2020]12号2021023103483005052762017GGJS107

2024

10.16098/j.issn.0529-1356.2024.05.013

解剖学报
中国解剖学会

解剖学报

CSTPCD
影响因子：0.462
ISSN：0529-1356
年,卷(期)：2024.55(5)