Scutellarin regulates SDF-1/CXCR4 axis to protect myocardial injury in sepsis rats
Objective To investigate the effect of Scutellarin on myocardial injury in septic rats by regulat-ing the SDF-1/CXCR4 signaling pathway.Methods SD rats were separated into sham surgery group,model group,low-dose Scutellarin group,high-dose Scutellarin group,high-dose Scutellarin+AMD3100(CXCR4 inhibitor)group with 12 rats in each group.The cecal ligation and perforation method was applied to prepare a rat sepsis model;blood biochemical analyzer was applied to detect serum creatine kinase(CK),lactate dehydrogenase(LDH)and myocardial troponin Ⅰ(cTnⅠ)levels;ELISA method was applied to detect the levels of IL-6,IL-1β,and TNF-α in serum;TUNEL staining was applied to detect cardiomyocyte apoptosis;HE staining was applied to observe patholog-ical changes in myocardial tissue;commercialized reagent kits were applied to detect levels of GSH,SOD,and MDA;Western blot was applied to detect the expression of Bcl-2,Bax,SDF-1,and CXCR4 proteins in myocardial tissue.Results Compared with the sham surgery group,the myocardial tissue in the model group was severely dam-aged,the levels of serum CK,LDH,cTnⅠ,IL-6,IL-1β and TNF-α,myocardial cell apoptosis rate,the level of myocardial tissue MDA,and the protein level of Bax increased obviously,the levels of GSH,SOD,the protein lev-els of Bcl-2,SDF-1,and CXCR4 in myocardial tissue reduced obviously(P＜0.05);compared with the model group,the myocardial tissue damage of rats in the low and high dose Scutellarin groups was obviously reduced,the levels of serum CK,LDH,cTnⅠ,IL-6,IL-1β and TNF-α,myocardial cell apoptosis rate,the level of myocardial tissue MDA,and the protein level of Bax reduced obviously,the levels of GSH,SOD,the protein levels of Bcl-2,SDF-1,and CXCR4 in myocardial tissue increased obviously(P＜0.05);compared with the high-dose Scutellarin group,the myocardial tissue damage of rats in the high-dose Scutellarin+AMD3100 group was obviously increased,the levels of serum CK,LDH,cTnⅠ,IL-6,IL-1β and TNF-α,myocardial cell apoptosis rate,the level of myocardi-al tissue MDA,and the protein level of Bax increased obviously,the levels of GSH,SOD,the protein levels of Bcl-2,SDF-1,and CXCR4 in myocardial tissue reduced obviously(P＜0.05).Conclusion Scutellarin can reduce inflam-matory response and oxidative stress in septic rats by activating the SDF-1/CXCR4 signaling pathway,and alleviate myocardial damage in septic rats.
NETL
NSTL
万方数据
