Exploration of the mechanism of sevoflurane on malignant biological activity of gastric cancer cells based on miR-34a/HMGB1 axis
Objective To explore the effect of sevoflurane on the malignant biological activity of gastric can-cer cells based on the miR-34a/HMGB1 axis.Methods The gastric cancer cells were divided into groups including the NC group,SEV-1.7％group,SEV-3.4％group,and SEV-5.1％group.Additionally,there were groups including the miR-34a group,SEV-5.1％+anti-miR-34a group,SEV-5.1％+pcDNA-HMGB1 group,and SEV-5.1％+pcDNA-HMGB1+miR-34a group.Cell proliferation was assessed using the CCK-8 assay,while cell invasion and migration capabilities were assessed using the Transwell chamber assay.The expression of miR-34a was measured using qRT-PCR,and the expression of HMGB1 protein in cells was detected using Western blot analysis.The targeting relation-ship between miR-34a and HMGB1 was investigated using a dual luciferase reporter system experiment.Results Compared to the GES-1 cell line,miR-34a expression was downregulated(0.23±0.03)and HMGB1 protein expres-sion was upregulated(0.85±0.10)in gastric cancer SGC-7901 cells(P＜0.05).Compared to the NC group,miR-34a expression increased and cell viability,cell invasion,and HMGB1 protein expression decreased in the SEV-1.7％group,SEV-3.4％group,and SEV-5.1％group(P＜0.05).Compared to the SEV-5.1％group,cell viability(92.46％±8.27％,96.71％±7.52％in 24 h and 48 h,respectively),cell invasion(126.42±10.84),and HMGB1 protein expression(0.80±0.09)significantly increased in the SEV-5.1％+anti-miR-34a group(P＜0.05).When trans-fecting HMGB1-WT into cells,the luciferase activity of the miR-34a group(0.34±0.05)was significantly lower than that of the miR-NC group(P＜0.05).Compared to the SEV-5.1％group,cell viability(90.18％±8.06％,82.47％± 7.21％in 24 h and 48 h,respectively),cell invasion(122.18±10.62),and HMGB1 protein expression(0.81± 0.10)significantly increased in the SEV-5.1％+pcDNA-HMGB1 group(P＜0.05).Compared to the SEV-5.1％+pcD-NA-HMGB1 group,cell viability(65.33％±6.02％,28.41％±3.26％in 24 h and 48 h,respectively),cell invasion(68.33±6.09),and HMGB1 protein expression(0.34±0.04)significantly decreased in the SEV-5.1％+pcDNA-HMGB1+miR-34a group(P＜0.05).Conclusion Sevoflurane can inhibit the proliferation and invasion of gastric cancer cells by regulating the miR-34a/HMGB1 axis.
Gastric cancer cellsSevofluraneMiR-34a/HMGB1 axisProliferationInvasion
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