Comparing the effects of gentle handling and rotating bar sleep deprivation methods on epi-sodic fear memory in female mice
Objective To explore the effects of gentle handling and rotating bar sleep deprivation methods on episodic fear memory in female mice.Methods Fifteen C57BU6J female mice were randomly divided into con-trol group,gentle handling sleep deprivation group and rotating bar sleep deprivation group,with 5 mice in each group.Among them,the gentle handling sleep deprivation group was established by using gentle handling method,while the rotating bar sleep deprivation group was established by using Pinnacle automatic sleep deprivation system,and the control group stayed in the original cage.The modeling time was from 8 a.m.to 5 p.m.,which lasted for 9 hours,and mice were free to access food and water during this period.On the day before sleep deprivation,mice were put into the training box of four-station conditioned fear system to complete training and learning.After sleep de-privation on the second day,the mice were immediately tested for episodic fear associated memory.Results During the training session on the first day,the freezing time of the mice gradually increased with constant foot shocks,con-firming the effectiveness of unconditioned stimulation.Furthermore,there was no statistically significant difference in the percentage of freezing time among the control group,the gentle handling sleep deprivation group,and the rotat-ing bar sleep deprivation group during the training session(t=1.127,P＞0.05).After acute sleep deprivation on the second day,the proportion of 5 minutes freezing time in total time of the episodic fear test in the control group,the gentle handling sleep deprivation group and the rotating bar sleep deprivation group were(50.85±8.92)％,(28.95± 12.45)％and(43.85±10.52)％,respectively.Compared with the control group,the 5 minutes total freezing time of the gentle handling sleep deprivation group showed significant reduction(t=3.227,P＜0.05);while there was no sta-tistical difference in the freezing time between the rotating bar sleep deprivation group and the control group(t=1.032,P＞0.05).There was also no significant difference between gentle handling sleep deprivation group and rotat-ing bar sleep deprivation(t=2.195,P＞0.05).Conclusions Acute sleep deprivation induced by gentle handling im-paired episodic fear memory in female mice,whereas no such impairment was observed in mice induced by rotating bar sleep deprivation.
Sleep deprivationCognitionEpisodic fear memoryFemale miceAnimal model
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