miR-106a regulates the biological behavior of gastric cancer cells through the regulation of PI3K/PDK1/AKT pathway
Objective To investigate the effects of miR-106a on the biological behavior of gastric cancer cells and its mechanism.Methods Human gastric cancer cell lines of AGS were selected and cultured into 27 sam-ples.All the samples were randomly divided into three groups:miR-106a inhibitor,miR-mimic and miR-NC,which were treated with miR-106a inhibitor,miR-106a mimic and placebo respectively.Cell survival rate,cell cycle,cell invasion,migration,caspase activity,relative expression levels of Bax,Bcl-2,Casepase-3 protein,and relative ex-pression levels of p85β,p-PDK1 and p-AKT protein were observed in each group.Results Compared with miR-NC,AGS cell activity of miR-106a inhibitor group was decreased(15.01±0.97 vs 69.82±2.31)(P＜0.01).The pro-portion of G0/G1 phase cells in AGS cells of miR-106a mimics group decreased(P＜0.05)(17.33±1.04 vs 58.24± 0.82).The proportion of G2/M and S phase cells increased(50.11±1.12 vs 35.64±1.07)(31.56±0.92 vs 9.24±0.25).The proportion of AGS cells in G0/G1 phase was increased(78.43±1.12 vs 58.24±0.82)(P＜0.05)after miR-106a in-hibitor treatment.The proportion of G2/M and S phase cells decreased(33.65±0.99 vs 35.64±1.07)(19.78±0.84 vs 9.24±0.25)(P＜0.01);Compared with miR-NC,the migration and invasion ability of AGS cells in miR-106a mimics group was enhanced.The migration and invasion ability of AGS cells were decreased vs miR-106a inhibitor group(P＜0.01).Compared with miR-NC,the activities of caspase-3,caspase-8 and caspase-9 of AGS cells in miR-106a mimics group were decreased.The activities of caspase-3,caspase-8 and caspase-9 of AGS cells in miR-106a inhibi-tor group were increased(P＜0.05).Compared with miR-NC,the relative expression levels of Bax and Casepase-3 in AGS cells of miR-106a mimics group were decreased(0.69±0.07 vs 1.48±0.15)(0.37±0.04 vs 0.91±0.09).The rela-tive expression of Bcl-2 protein was increased(1.53±0.12 vs 0.94±0.09).The relative expression levels of Bax and Casepase-3 in AGS cells of miR-106a inhibitor group were increased(2.07±0.21 vs 1.48±0.15)(1.23±0.12 vs 0.91± 0.09).The relative expression of Bcl-2 protein was decreased(P＜0.05)(0.65±0.07 vs 0.94±0.09).Compared with miR-NC.The relative expression levels of p85β,p-PDK1 and p-AKT in AGS cells of miR-106a mimics group were increased(1.24±0.12 vs 0.94±0.09)(2.13±0.23 vs 1.01±0.10)(1.14±0.11 vs 0.72±0.06).The relative expression levels of p85β,p-PDK1 and P-Akt in AGS cells of miR-106a inhibitor group were decreased(0.69±0.07 vs 0.94± 0.09)(0.75±0.07 vs 1.01±0.10)(0.53±0.05 vs 0.72±0.06)(P＜0.05).Conclusion The expression of miRNA-106a can regulate the biological behavior of cervical cancer cells through the regulation of PI3K/PDK1/AKT path-way,including reducing the viability,migration and invasion of cancer cells,and inducing cell cycle arrest of can-cer cells.Inhibiting the expression of miRNA-106a may be a new therapeutic target for patients with gastric cancer.
Human gastric cancer cell lines of AGSmiR-106aPhosphatidylinositol kinase(PI3K)Phosphate inositol dependent protein kinase-1(PDK1)Protein kinase B(AKT)Biological behavior of cancer cells
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