The effect of lncRNA CASC2 on proliferation,invasion and metastasis of thyroid cancer
Objective To explore the effect of cancer susceptibility candidate gene 2(CASC2)on the pro-liferation,invasion,and metastasis of thyroid cancer cell line(TPC-1)cells.Methods Collect 83 thyroid cancer patients who underwent surgical treatment in our hospital from January 2020 to December 2022,and prepare paraf-fin specimens of thyroid cancer and adjacent tissues.Real time fluorescence quantitative polymerase chain reaction(RT PCR)was used to detect the expression levels of CASC2 mRNA and miR-155-5p RNA in the cancer and adja-cent tissues.TPC-1 cells were randomly divided into plasmid group,empty group,and blank group.The plasmid group was transfected with CASC2a using liposome transfection reagent;The empty group was transfected with empty plasmids,while the blank group was not treated.The CCK-8 method was used to detect cell proliferation,while the Transwell invasion experiment was used to detect cell migration and invasion.Results The expression of lncrna-casc2 in cancer tissues(1.23±0.08)was significantly lower than that in adjacent tissues(2.16±0.11);However,the expression of mir-155-5p(2.18±0.16)was significantly higher than that of the adjacent tissues(1.46±0.10)(P＜0.05).There were a significant difference in A450 values at 48 h(0.32±0.03,0.40±0.04,0.42±0.06,respectively),72 h(0.65±0.08,0.73±0.10,0.77±0.11,respectively)and 96 h(0.97±0.12,1.24±0.14,1.35±0.15,respectively)in the cell group(P＜0.05);And with the increase of time,the A450 value of the three groups of cells increased.The migration and invasion abilities of the three groups were compared.The migration number(54.63±8.95)and invasion number(33.58±6.63)of the plasmid group were significantly lower than those of the empty group(89.37±10.04,72.37±9.72,respectively)and the blank group(93.21±10.22,75.82±9.62,respectively)(P＜0.05);However,there was no significant difference in the number of migration and invasion between the empty group and the blank group(P＞0.05).Conclusion LncRNA-CASC2 exhibits low expression in thyroid cancer tissue,while miR-150-5p is highly expressed in cancer tissue.Both factors are crucial for the occurrence and development of thyroid cancer;Moreover,lncRNA-CASC2 can inhibit the proliferation and migration ability of TPC-1 cells at the cellular level,but the key points and regulatory mechanisms of its anti-tumor effect need further exploration.
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