Preliminary study on the properties of novel breast cancer targeting small molecular peptides
Objective In this study,a new polypeptide molecule(AR)was designed and synthesized by solid phase synthesis method to investigate its targeting and biological safety for breast cancer.Methods A variety of breast cells were used as models to investigate the cell uptaken specificity of the fluorescent polypeptide molecular probe and the effect on the cell cycles by confocal microscopy and flow cytometry.Cytotoxicity analysis was per-formed by CCK8 to confirm the biosafety of the probe.Results The results of fluorescence and flow cytometry showed that the fluorescence intensity of MCF-7 cells was significantly higher than that of the breast epithelial cells MCF-10A and other types of breast cancer cells,identifying a good targeting specificity and low cytotoxicity of the probe on MCF-7 cells.CCK8 results showed that the cell viability of MCF-7 cells treated with different concentra-tions of AR-FITC was still higher than 80％,indicating that a low cytotoxicity of the probe.Cell cycle showed that the S-phase cells of MCF-7 cells in the experimental group and the control group were 12.45％±0.75％and 15.35％±0.35％,respectively,which was not statistically significant(P＞0.05),identifying that AR had no significant effect on the cell cycle of MCF-7 cells.Conclusion The novel peptide has good targeting and biosafety,and is a promis-ing molecular probe for breast cancer.
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