Effects of BMP4/FBN1 on the proliferation and invasiveness of spinal cord ependymoma based on TGF-βpathway
Objective To investigate the effect of BMP4/FBN1 on the proliferation and invasiveness of spi-nal cord ependymoma(SCE)based on TGF-β pathway.Methods Tumor tissues and normal tissues of SCE pa-tients were collected and divided into control group and case group.Cancer cells were cultured from SCE patients and the expression of FBN1 or BMP4 protein was knocked down with lentivirus gene carrying FBN1 shRNA and BMP4 shRNA.The expression levels of FBN1 and BMP4 genes and proteins in tumor tissue samples were detected by qRT-PCR and Western blot.The expression level of TGF-β gene was detected by qRT-PCR in tumor cells with low expression of BMP4 and FBN1.Effects of FBN1 or BMP4 protein knockdown in cancer cells on the proliferation and invasion were further examined by CCK-8 cell proliferation assay and Transwell invasion assay.Results Com-pared with the control group,BMP4 and FBN1 gene expression(P＜0.05,P＜0.01,respectively),and protein expres-sion were also significantly increased in the case group(P＜0.01).Knockdown of FBN or BMP4 protein not only down-regulated the expression of TGF-β gene in SCE cells(P＜0.05,P＜0.01,respectively),but also inhibited the prolifer-ation and invasion ability of SCE cells(P＜0.05,P＜0.01,respectively).Conclusion Based on TGF-β pathway,this study investigated the influence of BMP4/FBN1 on the proliferation and invasiveness of SCE,suggesting that there may be interactions between FBN1,BMP4 and TGF-β.Here we provided a useful theoretical basis for clinical treat-ment of SCE.
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