Protective effect and mechanism analysis of remazolam on nerve injury in sepsis
Objective To analyze the protective effect of remazolam on nerve injury in sepsis and its mecha-nism.Methods 27 adult male rats were randomly divided into control group,model group and study group.Sham operation was performed in the control group,sepsis nerve injury model was established in the model group,and the study group was given intraperitoneal injection of remazolam.The results of water maze experiment were observed and brain pathological changes,neuronal apoptosis rate,levels of IL-6,IL-10,TNF-α,mRNA of CHOP,TATF4,XBP1 and protein of IκBα,IKK,NF-κB were detected.Results There was no statistically significant difference in the results of water maze experiment among all groups before surgery(P＞0.05),but there were statistically signifi-cant differences in escape latency and number of platform crossing among all groups after surgery(P＜0.05).The es-cape latency of control group was lower than that of model group and research group,and the number of platform crossing was higher than that of model group and research group.The escape latency of study group was lower than that of model group,and the number of platform crossing was higher than that of model group(P＜0.05).HE stain-ing showed that the brain tissue of the control group had no obvious pathological changes,while the pathological changes of the model group were serious.The pathological changes of the study group were improved compared with the model group.The neuronal apoptosis rate was significantly different among all groups(P＜0.05),and the control group was lower than the model group and the research group.The study group was lower than the model group(P＜0.05).The levels of IL-6,IL-10 and TNF-α were significantly different among all groups(P＜0.05).The levels of IL-6,IL-10 and TNF-α in control group were lower than those in model group and research group.The levels of IL-6,IL-10 and TNF-α in study group were lower than those in model group(P＜0.05).The mRNA levels of CHOP,TATF4 and XBP1 were significantly different among all groups(P＜0.05).The mRNA levels of CHOP,TATF4 and XBP1 in control group were lower than those in model group and research group.CHOP,TATF4 and XBP1 mRNA in study group were lower than those in model group(P＜0.05).The relative expression of IκBα,IKK,NF-κB pro-tein in all groups had statistical significance(P＜0.05),and the relative expression of IκBα,IKK,NF-κB protein in control group was significantly lower than that in model group and research group.The relative expressions of IκBα,IKK and NF-κB proteins in the study group were significantly lower than those in the model group(P＜0.05).Conclusion Remazolam can achieve neuroprotective effect by inhibiting endoplasmic reticulum stress,neuroinflam-mation and nerve cell apoptosis in sepsis rats,and reduce nerve injury in sepsis rats.The mechanism may be related to IKK/NF-κB signaling pathway.
RemazolamSepsisNerve injuryInhibitor of kappa B kinase/Nuclear factor kappa-B(IKK/NF-κB)InflammationApoptosisRat
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