Effect of CircFNDC3B on the malignant biological behavior of gastric cancer cells by regulating the miR-107/HMGA1 axis
Objective To investigate the impact of circular RNA FNDC3B(CircFNDC3B)on the malig-nant biological behaviors of gastric cancer(GC)cells by regulating the micro RNA-107/high mobility group protein A1(miR-107/HMGA1)axis.Methods Normal gastric epithelial cells GES-1 and human gastric cancer cell lines AGS,HCG27,MKN28,BGC-823 were cultured in vitro,RT-qPCR was applied to detect the expression of CircF-NDC3B,miR-107,and HMGA1,and to screen the optimal cell line.Double Luciferase reporter gene experiment was applied to verify the targeting relationship between miR-107 and CircFNDC3B,HMGA1;BGC-823 cells were selected for transfection and grouped into si-NC group,si-CircFNDC3B group,si-CircFNDC3B+NC inhibitor,si-Cir-cFNDC3B+miR-107 inhibitor group,miR-NC group,miR-107 mics group,miR-107 mics+pcDNA,and miR-107 mics+HMGA1 group,MTT method was applied to detect cell proliferation;Transwell was applied to detect cell mi-gration and invasion;flow cytometry was applied to detect cell apoptosis;Western blot was applied to detect the ex-pression of Ki67,Cyclin D1,Bcl-2,cleaved caspase3,E-cadherin,and N-cadherin proteins.Results In GC cell lines,the expression of CircFNDC3B and HMGA1 obviously increased,while the expression of miR-107 obviously decreased(P＜0.05);the results of double luciferase reporter gene experiment showed that miR-107 had a targeting relationship with CircFNDC3B and HMGA1;silencing the expression of CircFNDC3B or overexpressing miR-107 was able to obviously inhibit cell proliferation,migration,invasion,and EMT,and promote cell apoptosis(P＜0.05);inhibiting the expression of miR-107 or overexpressing HMGA1 was able to reverse the inhibitory effects of si-lencing CircFNDC3B or overexpressing miR-107 on the proliferation,migration,invasion,and EMT of BGC-823 cells(P＜0.05).Conclusion CircFNDC3B is highly expressed in GC cells,and silencing CircFNDC3B inhibits the malignant biological behavior of gastric cancer cells by regulating the miR-107/HMGA1 axis.
Gastric cancer cellsCyclic RNA FNDC3BMicro RNA-107/high mobility group protein A1Malignant biological behavior
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