Objective To explore the effect of Dahuang zhechong pill on the inflammatory injury of aorta in atherosclerotic(AS)rats by regulating IL-1β/NF-κB/NLRP3 signaling pathway.Methods Forty rats were divided into normal control group(NS),model group,Di'ao Xinxuekang intervention group(positive group)and Dahuang zhechong pill intervention group(Dahuang zhechong pill group),with 10 rats in each group.Except for the normal group,the other three groups of mice all built AS models.The pathological sections of aorta were observed by oil red staining,and serum lipid levels[triglyceride(TG),high density lipoprotein cholesterol(HDL-C),total cholesterol(TC)and low density lipoprotein cholesterol(LDL-C)],serum inflammatory indexes[interleukin(IL)-6,IL-1βand tumor necrosis factor(TNF)]were detected by EILSA method.The peripheral blood Th17/Treg cell level was detected by flow cytometry,and the mRNA and protein expression levels of IL-1β/NF-κB/NLRP3 signaling pathway were detected by RT-qPCR and Western blot,respectively,and the AI index was calculated.Results Intima thick-ening,plaque deposition,discontinuity and infiltration of a large number of inflammatory cells were found in arterial tissues of rats in other groups except NC group.Among them,the injury of arterial tissue in the model group is the most obvious,and the positive group and Dahuang zhechong pill group are obviously improved compared with the model group,and the arterial tissue structure is similar to that in the NC group.The levels of serum TC(9.82 mmol/L±1.46 mmol/L),TG(9.82 mmol/L±1.46 mmol/L),LDL-C(2.93 mmol/L±0.22 mmol/L),IL-6(97.65 ng/L±9.11 ng/L),TNF-α(93.21 ng/L±11.17 ng/L)and peripheral blood Th 17(3.56±0.34)％and Treg cells(4.41±0.38)％in the mod-el group were higher than those in the NC group(3.71 mmol/L±0.57 mmol/L),(6.47 mmol/L±0.92 mmol/L),(0.94 mmol/L±0.15 mmol/L),(16.52 ng/L±2.76 ng/L),(5.45 ng/L±0.84 ng/L),(1.83％±0.16％),(7.72％±0.73％),re-spectively]and the level of HDL-C(2.92 mmol/L±0.31 mmol/L)was lower(0.95 mmol/L±0.08 mmol/L)(all P＜0.05).Compared with the model group,in Dahuang zhechong pill group and positive group,the levels of serum TC(3.92 mmol/L±0.72 mmol/L,3.71 mmol/L±0.65 mmol/L),TG(6.71 mmol/L±0.83 mmol/L,6.83 mmol/L±0.72 mmol/L),LDL-C(1.15 mmol/L±0.11 mmol/L,1.11 mmol/L±0.13 mmol/L,IL-6(19.83 ng/L±2.55 ng/L,18.51 ng/L±3.72 ng/L)and TNF-α(17.08 ng/L±1.72 ng/L,15.92 ng/L±1.56 ng/L),and the levels of peripheral blood Th 17(2.11％±0.25％),2.06％±0.22％)and Treg cells(7.45％±0.76％),(7.31％±0.72％)were lower,and HDL-C(2.18 mmol/L±0.72 mmol/L,2.23 mmol/L±0.61 mmol/L)was higher,which was close to that in NC group(all P＜0.05).Compared with NC group,the AI index of model group,positive group and Dahuang zhechong pill group increased significantly NC group(0.35±0.08,9.71±2.04,1.21±0.15 and 0.83±0.17,respectively)(all P＜0.05),and the AI index of posi-tive group and Dahuangzhechong pill group was lower than that of model group(all P＜0.05).The expression levels of IL-1 β,NF-κB,NLRP3 mRNA and signal pathway protein in peripheral blood of rats in model group were signifi-cantly higher than those in NC group,positive group and rhubarb sting group(all P＜0.05).Conclusion Dahuang zhechong pill can obviously improve the inflammatory injury of aorta in atherosclerotic rats,which may be achieved by inhibiting IL-1 β/NF-κB/NLRP3 signaling pathway.
Dahuang zhechong pillAtherosclerosisIL-1β/NF-κB/NLRP3 signal pathwayAortaInflammatory injury
NETL
NSTL
万方数据
