Effect of CGF combined with biological bone repair materials on osteogenesis of alveolar defect and mecha-nism analysis of CT evaluation
Objective To explore the effect of concentrated growth factor(CGF)combined with Bio-Oss bone powder,a biologic bone repair material,on osteogenesis and CT evaluation of alveolar defect repair.Meth-ods Eight Beagle dogs(male and female)from 8～12 months of age were selected as the study subjects,and each dog was established as an animal model of alveolar crestal fissure by artificial fissure creation in the left and right side of the maxilla symmetrically,and the animal models were randomly given autogenous bone iliac cancellous re-pair(control group)and CGF+Bio-Oss mixture repair(experimental group)on the left and right side,respectively,with appropriate pressure and adequate filling to ensure that the mucosal flap was free of The sutures were tightly closed with no tension.The bone resorption rate was calculated by CT measurement at 3 and 6 months for each group,respectively.The animals were executed at month 6 after surgery,and the bone between the distal middle of the central incisor and the proximal middle of the cuspid was intercepted and fixed in 4％paraformaldehyde for 2 weeks,during which Micro CT was taken to measure the osteogenic quality[bone volume fraction(BV/TV),bone trabecular thickness(Tb.Th),and trabecular separation(Tb.Sp)].Sections were made according to pathological standards,HE stained,microscopically observed and photographed and recorded.The expressions of osteoblast marker genes,osteoblast differentiation genes and osteoblast apoptosis-related genes were compared between the two groups 3 months and 6 months after operation.Results The bone resorption rates of the experimental group and the control group were greater than that of the 3-month postoperative period(experimental group:18.95％±2.27％com-pared to 11.26％±1.52％,control group:21.99％±3.01％compared to 15.91％±2.36％)(t=5.135,2.963,respective-ly,both P＜0.05);the bone resorption rates of the experimental group in the 3-month postoperative period were less than that of the control group(t=2.869,P＜0.05);the difference in bone resorption rate at 6 months postoperatively between the two groups was not statistically significant(t=1.803,P＞0.05).BV/TV was greater in the experimental and control groups at 6 months postoperatively than at 3 months postoperatively(experimental group:0.46±0.05 vs.0.39±0.05,control group:0.40±0.04 vs.0.34±0.04),and Tb.Sp was less than at 3 months postoperatively(experi-mental group:0.14±0.02 vs.0.18±0.02,control group:0.17±0.02 than 0.21±0.03)(t=2.215,2.304,2.311,2.237,respectively,P＜0.05);BV/TV was greater than that of the control group in the experimental group at 3 months postoperatively,and Tb.Sp was less than that of the control group(t=2.209,2.372,respectively,P＜0.05);and the differences in the BV/TV and Tb.Sp between the two groups at 6 months postoperatively were were not statis-tically significant(t=2.095,2.353,respectively,P＞0.05);there was no statistical difference in Tb.Th between the two groups at 3 and 6 months after surgery(t=2.043,2.039,respectively,P＞0.05).Under a low magnification mi-croscope,the bone tissue samples from both the experimental and control groups exhibited a distinct layer of mature bone cortex,which was tightly packed with internal bone trabeculae,which were arranged in a haphazard manner and were spaced by an adipose tissue rich in blood constituents,which was enriched with blood cells such as erythro-cytes and leukocytes,and showed a structure similar to a honeycomb.Under high magnification,it was possible to observe more clearly that the osteoblasts were uniformly distributed in the bone,and between these active osteo-blasts,osteoclasts in a non-functional state were scattered.In addition,a relatively small number of osteoblasts were attached around the bone trabeculae,which were tightly adhered to the surface of the trabeculae,whereas the space between the trabeculae was filled by the blood cell-rich adipose tissues mentioned earlier,and vascular-like struc-tures could be observed at the junction of these adipose tissues,which implied that the blood supply to the bone tis-sues was adequate.At 3 and 6 months after surgery,the expressions of OC,OPG and Runx2 genes in the study group were higher than those in the control group(t=2.526,3.046,3.660,3.941,2.933,3.145,respectively,P＜0.05).At 3 and 6 months after surgery,PCNA and ki67 gene expressions in the study group were higher than those in the control group,while BAX gene expressions were lower than those in the control group(t=8.245,8.481,10.492,8.515,5.643,6.903,respectively,P＜0.05).Conclusion CGF combined with Bio-Oss bone meal can achieve better results than autologous bone transplantation in repairing alveolar defects.It can not only effectively re-duce postoperative bone absorption rate,but also promote osteoblast differentiation and bone formation,and reduce abnormal apoptosis of osteoblasts.
Alveolar defectConcentrated growth factorBio-Oss bone powderRestoration
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