首页|甲磺酸乐伐替尼对猪胸导管内皮细胞增殖和侵袭的影响

甲磺酸乐伐替尼对猪胸导管内皮细胞增殖和侵袭的影响

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目的 研究甲磺酸乐伐替尼(Levatinib mesylate)对取自猪胸导管的淋巴管内皮细胞(LECs)增殖和侵袭的影响,探讨甲磺酸乐伐替尼对淋巴管生成作用的相关调控机制.方法 取健康猪的胸导管消化法进行LECs原代培养,用VEGFR-3(血管内皮生长因子-3)进行鉴定;采用EdU检测法检测甲磺酸乐伐替尼药物作用后LECs的增殖活力;采用Transwell侵袭实验检测甲磺酸乐伐替尼是否对LECs的侵袭能力有影响;Western blot(蛋白质印迹)法分别测定甲磺酸乐伐替尼药物作用后LECs的VEGFR-3靶蛋白的含量.结果 EdU结果显示,甲磺酸乐伐替尼药物作用后LECs增殖能力降低,当药物浓度达到10 nmol/L时,实验组EdU标记率(25±6)%明显低于对照组(49±9)%,LECs的增殖能力被甲磺酸乐伐替尼显著抑制(P<0.05);Transwell侵袭实验结果可见,LECs的侵袭能力能够被甲磺酸乐伐替尼抑制,当药物浓度达到10 nmol/L时,实验组LECs侵袭数量(66.33±8.93)低于对照组(138.67±7.02),LECs透膜数量明显减少(P<0.05);Western blot结果可见,甲磺酸乐伐替尼可以下调LECs的VEGFR-3蛋白表达,且随着药物浓度的增加,蛋白含量下调更为显著(P<0.05).结论 甲磺酸乐伐替尼可通过作用于VEGFR-3靶蛋白,从而抑制LECs增殖和侵袭.
Effect of lenvatinib mesylate on proliferation and invasion of endothelial cells in pig thoracic ducts
Objective To study the effect of levatinib mesylate on the proliferation and invasion of lymphat-ic endothelial cells(LECs)taken from pig thoracic ducts,and explore the relevant regulatory mechanisms of leva-tinib mesylate on lymphangiogenesis.Methods The thoracic duct digestion method of healthy pigs was used for pri-mary culture of LECs,and VEGFR-3(vascular endothelial growth factor-3)was used for identification;Using EdU detection method to detect the proliferation activity of LECs after the action of levatinib mesylate;Transwell invasion assay was used to detect whether the invasive ability of LECs after the action of levatinib mesylate;The Western blot method was used to determine the content of VEGFR-3 target protein in LECs after the action of levatinib mesylate.Results The EdU results showed that the proliferation ability of LECs was reduced after the action of levatinib me-sylate.When the drug concentration reached 10 nmol/L,the EdU labeling rate in the experimental group(25%±6%)was significantly lower than that in the control group(49%±9%),and the proliferation ability of LECs was significantly inhibited(P<0.05);The results of the Transwell invasion experiment showed that the invasive ability of LECs cells could be inhibited by lovatinib mesylate.When the drug concentration reached 10 nmol/L,the number of LECs invading the experimental group(66.33±8.93)was lower than that of the control group(138.67±7.02),the number of LECs membranes significantly decreased(P<0.05);Western blot results showed that levatinib mesylate can downregulate the expression of VEGFR-3 protein in LECs,and the protein content was more significantly down-regulated with the increase of drug concentration(P<0.05).Conclusion Levatinib mesylate can inhibit the prolifer-ation and invasion of LECs by acting on the VEGFR-3 target protein.

Lymphatic endothelial cellsLevatinib mesylateVascular endothelial growth factor(VEGF)ProliferationInvasion

赵红博、熊桂宏、邸子轩、李沐涵、李明秋

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牡丹江医学院人体解剖学教研室,黑龙江牡丹江 157011

淋巴管内皮细胞 甲磺酸乐伐替尼 血管内皮生成因子 增殖 侵袭

黑龙江省省属高等学校基本科研业务费科研项目牡丹江医学院研究生导师科研专项计划

2017-KYYWFMY-0677YJS2X2022009

2024

解剖学研究
广东省解剖学会 中国解剖学会

解剖学研究

CSTPCD
影响因子:0.327
ISSN:1671-0770
年,卷(期):2024.46(4)
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