Effect of lenvatinib mesylate on proliferation and invasion of endothelial cells in pig thoracic ducts
Objective To study the effect of levatinib mesylate on the proliferation and invasion of lymphat-ic endothelial cells(LECs)taken from pig thoracic ducts,and explore the relevant regulatory mechanisms of leva-tinib mesylate on lymphangiogenesis.Methods The thoracic duct digestion method of healthy pigs was used for pri-mary culture of LECs,and VEGFR-3(vascular endothelial growth factor-3)was used for identification;Using EdU detection method to detect the proliferation activity of LECs after the action of levatinib mesylate;Transwell invasion assay was used to detect whether the invasive ability of LECs after the action of levatinib mesylate;The Western blot method was used to determine the content of VEGFR-3 target protein in LECs after the action of levatinib mesylate.Results The EdU results showed that the proliferation ability of LECs was reduced after the action of levatinib me-sylate.When the drug concentration reached 10 nmol/L,the EdU labeling rate in the experimental group(25%±6%)was significantly lower than that in the control group(49%±9%),and the proliferation ability of LECs was significantly inhibited(P<0.05);The results of the Transwell invasion experiment showed that the invasive ability of LECs cells could be inhibited by lovatinib mesylate.When the drug concentration reached 10 nmol/L,the number of LECs invading the experimental group(66.33±8.93)was lower than that of the control group(138.67±7.02),the number of LECs membranes significantly decreased(P<0.05);Western blot results showed that levatinib mesylate can downregulate the expression of VEGFR-3 protein in LECs,and the protein content was more significantly down-regulated with the increase of drug concentration(P<0.05).Conclusion Levatinib mesylate can inhibit the prolifer-ation and invasion of LECs by acting on the VEGFR-3 target protein.