Resveratrol promotes ovarian cancer cell apoptosis and enhances its chemotherapy sensitivity by mediating GAL-3 expression
Objective To investigate the effects of resveratrol(RES)on apoptosis and chemotherapy sensi-tivity of ovarian cancer cells and its mechanism.Methods The samples were ovarian cancer resistant cell line SKOV3 cell lines,which were divided into 3 groups with 11 cells in each group.The control group was routinely cul-tured,the cisplatin group was given 10 µmol/L cisplatin intervention,and the RES+cisplatin group was given µmol/L 50 μmol/L RES+10 µmol/L cisplatin intervention.Cell viability was detected by CCK-8 assay,cell proliferation was detected by BrdU assay,apoptosis was detected by Annexin V-FITC/PI double staining apoptosis assay,caspase-3 activity was detected by colorimetric assay,cell migration ability was detected by Transwell cell migration assay,and cell invasion assay was detected by invasion assay.Western blot analysis of caspase-3,GAL-3,p-Akt,AKT,Iκβα,P65,Bcl-2,p-IKKα/β protein relative expression.Results The cell viability and cell proliferation rate in RES+cisplatin group and cisplatin group were significantly lower than those in control group,and the rate in RES+cisplatin group was significantly lower than that in cisplatin group(62％±14％,74％±16％,and 98％±16％;59％±16％,76％±17％and 97％±17％,respectively).The apoptosis rate of RES+cisplatin group and cisplatin group was significantly higher than that of control group,and that of RES+cisplatin group was significantly higher than that of cisplatin group(64.29％±13.14％,47.48％±9.52％,14.72％±3.03％,respectively)(P＜0.05).The activity and relative expression of caspase-3 in RES+cisplatin group were significantly higher than those in cisplatin group and control group(P＜0.05).The amount of cell migration and cell invasion in RES+cisplatin group and cisplatin group were significantly lower than those in control group,and RES+cisplatin group was significantly lower than that in cis-platin group(P＜0.05);The relative expressions of GAL-3 and p-Akt protein in RES+cisplatin group were signifi-cantly lower than those in cisplatin group and control group(P＜0.05).There was no significant difference in the ex-pression of AKT among all groups(P＜0.05).The relative expressions of p-IKKα/β,P65,Bcl-2 and protein in RES+cisplatin group and cisplatin group were significantly lower than those in control group,and RES+cisplatin group was significantly lower than that in cisplatin group(P＜0.05);The relative expression of I Kβα protein in RES+cisplatin group and cisplatin group was significantly higher than that in control group,and RES+cisplatin group was significantly higher than that in cisplatin group(P＜0.05).Conclusion The apoptosis induced by RES may be relat-ed to the decreased level of GAL-3.The mechanism of its action may be to inhibit the proliferation and invasion of ovarian cancer(OC)cells and enhance their sensitivity to cisplatin by regulating apoptosis and expression of transfer-related proteins.
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