The expression of miR-551 in thyroid cancer patients and the mechanism of targeting ERBB4 on the growth of SW579 cell transplanted tumor
Objective To explore the expression of miR-551 in thyroid cancer patients and the effect of tar-geted ERBB4 on the growth of SW579 cell transplantation tumors.Method 100 cases of papillary thyroid cancer tissue and its paired adjacent tissues collected and preserved in the Department of Pathology of our hospital from Jan-uary 2019 to March 2022 were selected.SW579 cells were randomly divided into Control group,miR-NC group,miR-551 group,and miR-551+pcDNA-ERBB4 group.RT-PCR was used to detect the expression of miR-551 in thyroid cancer tissue and cells,and to analyze the correlation between miR-551 expression and clinical pathological features in thyroid cancer tissue;CCK-8,Transwell method,and flow cytometry were used to detect cell proliferation,inva-sion,and apoptosis rates,respectively;Protein blotting was used to detect the expression of ERBB4 protein in cells;The luciferase activity gene report detects the targeting relationship between miR-551 and ERBB4.Establish a nude mouse subcutaneous transplant tumor model and divide it into miR-NC group and miR-551 group.Compare the tumor volume between two groups of nude mice;TUNEL was used to detect the apoptosis rate of tumor tissue cells.Result The expression of miR-551 in thyroid cancer tissue was lower than that in adjacent tissues(P＜0.05).Com-pared with thyroid epithelial cells(1.00±0.10),the expression of miR-551 in thyroid cancer cells was significantly reduced(0.32±0.04)(P＜0.05).Compared with the control group,the expression of miR-551(1.28±0.13)and apoptosis rate(25.72±1.78)in the miR-551 group cells were significantly increased,while 24 h and 48 h cell viabil-ity(52.38±5.46,32.29±3.17,respectively),cell invasion number(81.06±6.32),and ERBB4 protein(0.46±0.05)in the cells were significantly reduced(P＜0.05);Compared with the miR-551 group,the miR-551+pcDNA-ERBB4 group showed a significant decrease in miR-551 expression and apoptosis rate(7.69±0.82),while 24 h and 48 h cell viability(85.11±7.61,71.56±6.82,respectively),cell invasion number(136.82±7.54),and expression of ERBB4 protein in cells(0.84±0.08)were significantly increased(P＜0.05).Compared with the miR-NC group(1.00±0.10),the luciferase activity of the miR-551 group significantly decreased(0.34±0.04)when transfected with wild-type ERBB4(ERBB4-WT)(P＜0.05).Compared with the miR-NC group,the miR-551 group showed a signifi-cant decrease in tumor volume and an increase in cell apoptosis rate in tumor tissue(51.62±4.31)(P＜0.05).Con-clusion miR-551 is downregulated in thyroid cancer tissues.Overexpression of miR-551 can target and regulate ERBB4,inhibiting proliferation and invasion of thyroid cancer cells while inducing apoptosis.
Thyroid cancermiR-551Oncogene encoding human epidermal growth factor receptor(ERBB4)Transplanted tumorProliferationInvasionApoptosis
NETL
NSTL
万方数据
