Effect of vitexin on IL-1β-induced apoptosis of articular chondrocytes by regulating the PI3K/Akt pathway
Objective To investigate the effect of vitexin on interleukin-1β(IL-1β)-induced apoptosis of articular chondrocytes and its effects on the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)pathway.Methods Human articular chondrocytes were cultured in vitro and treated with 0-200 μmol/L vitexin to screen for the optimal concentration;human articular chondrocytes were separated into control group,IL-1β group,10 μmol/L vitexin group,50 μmol/L vitexin group,and vitexin+PI3K inhibitor(LY294002)group,CCK-8 method was ap-plied to detect cell proliferation in each group,flow cytometry was applied to detect cell apoptosis rate,ELISA was applied to detect the levels of IL-6 and tumor necrosis factor-α(TNF-α)in cell supernatant,thiobarbituric acid method was applied to determine the content of malondialdehyde(MDA)in cell supernatant,xanthine oxidation method was applied to determine the activity of superoxide dismutase(SOD)in cell supernatant,Western blot meth-od was applied to detect the expression of phosphorylated(p-)PI3K,PI3K,p-Akt,Akt,and cleaved cysteine con-taining aspartate proteolytic enzyme 3(C-Caspase-3)proteins in cells.Results Compared with the control group,the cell survival rate,SOD activity,and the expression levels of p-PI3K/PI3K(0.58±0.06 vs 0.91±0.09)and p-Akt/Akt(0.15±0.03 vs 0.69±0.08)proteins decreased in IL-1β group,the apoptosis rate(38.21％±3.26％vs 2.88％±0.64％),IL-6,TNF-α,MDA contents,and the expression level of C-Caspase-3 protein increased(P＜0.05);com-pared with the IL-1β group,the cell survival rate,SOD activity,and the expression levels of p-PI3K/PI3K(0.71±0.08,0.87±0.09 vs 0.58±0.06)and p-Akt/Akt(0.32±0.04,0.59±0.07 vs 0.15±0.03)proteins in the 10 and 50μmol/L vitexin groups gradually increased,the apoptosis rate(25.36％±2.94％,18.36％±1.95％vs 38.21％±3.26％),IL-6,TNF-α,MDA contents,and the expression level of C-Caspase-3 protein gradually decreased(P＜0.05);com-pared with the 50 μmol/L vitexin group,the cell survival rate,SOD activity,and the expression levels of p-PI3K/PI3K(0.61±0.07 vs 0.87±0.09)and p-Akt/Akt(0.19±0.03 vs 0.59±0.07)proteins in the vitexin+LY294002 group decreased,the apoptosis rate(26.71％±2.78％vs 18.36％±1.95％),IL-6,TNF-α,MDA contents,and the expres-sion level of C-Caspase-3 protein increased(P＜0.05).Conclusion Vitexin may inhibit IL-1β-induced apoptosis of articular chondrocytes by activating the PI3K/AKT pathway.
VitexinOsteoarthritisArticular chondrocytesApoptosisPhosphatidylinositol 3-ki-nase/protein kinase B
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