Neuroprotective effect of lidocaine on ischemic stroke rats by regulating the RhoA/ROCK signaling pathway
Objective To investigate the neuroprotective effect of lidocaine on ischemic stroke(IS)rats by regulating the RhoA/ROCK signaling pathway.Methods A model of IS rats was established by thread occlusion method and randomly separated into model group,lidocaine low(lidocaine-L,5 mg/kg),medium(lidocaine-M,10 mg/kg),high-dose(lidocaine-H,20 mg/kg)groups,and lidocaine-H+Lysophosphatidic acid(LPA)(20 mg/kg li-docaine+50 μmoUL LPA)group,rats that were separated but not inserted with nylon thread were in the sham opera-tion group,after the intervention,the neurological function score,cerebral infarction volume,and brain water con-tent of the rats were measured;brain tissue was isolated,and changes in neurons,neuronal apoptosis,and expres-sion of RhoA and ROCK proteins were observed.Results Compared to the sham group,Zea-Longa score(3.28±0.33,0.14±0.02),cerebral infarction volume(38.09±3.81,0.00±0.00),water content(84.11±2.39,62.95±0.65),apoptosis rate(39.51±3.96,5.14±0.5)in model group 2),RhoA(1.16±0.12,0.24±0.03),ROCK protein(2.15±0.22,0.77±0.08)were increased(P＜0.05);Compared to the model group,Zea-Longa score(2.37±0.24,1.52±0.16,0.87±0.09),cerebral infarction volume(27.42±2.76,16.64±1.68,8.22±0.83),water content(75.24±1.46,68.34±1.02,63.15)in lidocaine group±0.86),neuronal apoptosis rate(27.08±2.71,18.84±1.89,9.47±0.95),RhoA(0.78±0.08,0.52±0.06,0.26±0.03),ROCK protein(1.66±0.17,1.24±0.13,0.86±0.09)The expression was significantly decreased and there were differences between groups(P＜0.05);LPA reversed the protective effect of li-docaine-H on IS rats.Conclusion Lidocaine exerts neuroprotective effects on IS rats by inhibiting the RhoA/ROCK signaling pathway.
lidocaineIschemic strokeRhoA/ROCKNeuroprotectionAnimal model
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