首页|高频重复经颅磁刺激对血管性痴呆大鼠学习记忆能力的影响及机制

高频重复经颅磁刺激对血管性痴呆大鼠学习记忆能力的影响及机制

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目的 观察血管性痴呆(VD)大鼠学习记忆能力是否通过高频重复经颅磁刺激(HrTMS)发生改变,并探讨其作用机制.方法 Wistar大鼠83只,5只用于测定TMS静息运动阈值,另外78只大鼠随机分成假手术组、模型组和HrTMS组.用改良四血管闭塞法(4VO)制作全脑缺血再灌注模型;术后第3天开始给予HrTMS.大鼠空间学习记忆能力检测采用Morris水迷宫实验;观察海马形态学改变使用苏木精-伊红(HE)染色,免疫组化技术检测BDNF、MAP2蛋白.结果 与模型组逃逸潜伏期(56.67±0.72)s相比,HrT-MS组(52.51±0.70)s显著缩短(P＜0.05);在空间探索实验中,大鼠在60 s内穿过原有平台的次数,HrTMS组(2.13±0.30)次多于模型组(1.00±0.27)次(P＜0.05).HE染色显示,相比模型组,HrTMS组CA1区细胞坏死数量减少,形态学改变减轻.免疫组化提示,rTMS治疗14 d后,HrTMS组BDNF COD值(0.680±0.019)、MAP2 COD 值(0.456±0.020)与模型组的BDNF COD 值(0.606±0.019)、MAP2 COD 值(0.345±0.020)相比,表达增高(P＜0.05).结论 高频rTMS能上调BDNF、MAP2蛋白的表达,可能是其促进血管性痴呆大鼠学习记忆认知功能恢复的原因.
Effects of High-frequency repetitive transcranial magnetic stimulation on learning and memory in vas-cular dementia rats and its mechanism
Objective To observe the effect of high-frequency transcranial magnetic stimulation(HrTMS)on learning and memory abilities in vascular dementia(VD)rats and explore its mechanism.Methods 5 of 83 male Wistar rats were used for magnetic stimulation resting motion threshold measurement.The other 78 rats were divided into SHAM group,MODEL group,and HrTMS group by randomly.The whole brain ischemia-reperfusion model was made by modified four-vessel occlusion(4VO);HrTMS was administered on the 3rd day after surgery.Morris water maze test was used to detect the spatial learning and memory ability of rats;HE staining technique was used to ob-serve the morphological changes of hippocampus,BDNF and MAP2 proteins were detected by immunohistochemis-try.Results Compared with the escape latency of the model group(56.67 s±0.72 s),the HrTMS group(52.51 s±0.70 s)was significantly shortened(P＜0.05);In the space exploration experiment,the number of times rats crossed the original platform within 60 seconds was higher in the HrTMS group(2.13±0.30)than in the model group(1.00±0.27)(P＜0.05).HE staining showed that compared to the model group,the HrTMS group had a reduced number of cell necrosis and reduced morphological changes in the CA1 region.Immunohistochemical analysis showed that after 14 days of rTMS treatment,the expression of BDNF COD(0.680±0.019)and MAP2 COD(0.456±0.020)in the HrTMS group increased compared to the BDNF COD(0.606±0.019)and MAP2 COD(0.345±0.020)values in the model group(P＜0.05).Conclusion High frequency rTMS can upregulate the expression of BDNF and MAP2 pro-teins,which may be the reason why it promotes the recovery of learning,memory,and cognitive function in VD rats.

Vascular dementiaHigh frequency transcranial magnetic stimulationBrain-derived neuro-trophic factorMicrotubule-associated protein 2

赵嘉培、陈善佳、黄吉莉、何晓阔

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厦门市第五医院康复医学科,福建厦门 361101

血管性痴呆 高频经颅磁刺激 脑源性神经营养因子 微管相关蛋白2

福建省自然科学基金计划项目厦门市医疗卫生重点项目厦门市医疗卫生指导性项目医学科研计划项目

2022D0103502Z202340053502Z20214ZD12622023YJ001

2024

10.20021/j.cnki.1671-0770.2024.05.10

解剖学研究
广东省解剖学会 中国解剖学会

解剖学研究

CSTPCD
影响因子：0.327
ISSN：1671-0770
年,卷(期)：2024.46(5)