Mechanism of circFNDC3B regulation of macrophage polarization on apoptosis and GPNMB/CD44 axis in oral squamous cell carcinoma cells
Objective To investigate the mechanism of circFNDC3B-regulated macrophage polarization on apoptosis and GPNMB/CD44 axis in oral squamous cell carcinoma.Methods Transfect the overexpression circF-NDC3B plasmid into M0 macrophages and divide them into con group and circFNDC3B group.RT-PCR was used to detect the relative expression levels of CD16,TLR2,CD206,and CD14 in macrophages;Co culture macrophages from the con group and circFNDC3B group with OSCC cell line SCC-25,designated as M0 con group and M0 circF-NDC3B group;Transwell method for detecting cell invasion;Flow cytometry is used to detect the ability of cells to undergo apoptosis;Western blot was used to detect the expression of GPNMB and CD44 proteins in cells.Results Compared with the con group,the relative expression levels of M1 macrophage marker proteins CD16 and TLR2 in macrophages of the circFNDC3B group were reduced(P＜0.01);The relative expression levels of M2 macrophage marker proteins CD206 and CD14 were both increased(P＜0.01).Compared with the M0 con group,the M0 circF-NDC3B group showed a significant increase in the number of cell invasions and the expression of GPNMB and CD44 proteins in the cells,while the apoptosis rate was significantly reduced(P＜0.05).Conclusion Inhibition of circF-NDC3B promotes M1-type macrophage polarization,which promotes apoptosis in oral squamous cell carcinoma cells and inhibits activation of the GPNMB/CD44 axis.
Oral squamous cell carcinomaFibronectin type Ⅲ domain-containing protein 3B(FNDC3B)Circular RNAMacrophage polarizationApoptosisGPNMB/CD44 axis
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维普
