Resveratrol affects the malignant biological process of hepatocellular carcinoma by regulating SIRT1/STAT1 pathway
Objective To explore the effect of resveratrol on hepatocellular carcinoma and its related mech-anism,in order to provide new ideas for the treatment of hepatocellular carcinoma.Methods HepG2 cells were di-vided into control group,resveratrol low-dose group(RES-L,10 μmol/L),resveratrol high-dose group(RES-H,30μmol/L)and paclitaxel group(20 μmol/L).MTT assay,colony formation assay,Transwell assay and cell scratch assay were used to analyze the effect of resveratrol on the activity,invasion and migration ability and apoptosis rate of HepG2 cells.Real-time PCR and Western blot were used to analyze the effect of resveratrol on SIRT1/STAT1 sig-naling pathway.Results Compared with the control group,resveratrol could inhibit the viability of HepG2 cells(100.87％±5.78％,81.28％±3.25％,46.36％±8.05％,44.68％±7.11％in control group,RES-L,RES-H and pacli-taxel group,respectively).It also reduced the colony formation of HepG2 cells(102.52％±6.82％,88.36％±5.15％,30.26％±6.05％,26.38％±5.31％in control group,RES-L,RES-H and paclitaxel group,respectively)and inhibit-ed the invasion and migration of HepG2 cells,promoted the apoptosis of HepG2 cells(8.32％±0.72％,12.16％±3.05％,26.13％±1.25％,41.88％±6.81％)in control group,RES-L,RES-H and paclitaxel group,respectively.In addition,the protein expression levels of SIRT1 and STAT1 increased after resveratrol treatment compared with the control group.Conclusion Resveratrol can inhibit the malignant biological process of HepG2 cells,and this effect of resveratrol may be related to the activation of SIRT/STAT1 signaling pathway.
ResveratrolHepatocellular carcinomaSilent information regulator 1(SIRT)Signal transducers and activators of transcription(STAT1)
万方数据
维普
