Mechanism of miR-485-5p regulating the mek/erk signal pathway on the proliferation,invasion,and apop-tosis of hepatocellular carcinoma cells
Objective To investigate the mechanism of miR-485-5p regulating the MEK/ERK signaling pathway on the proliferation,invasion,and apoptosis of liver cancer cells.Methods Fifty liver cancer patients who underwent radical resection of liver cancer in our hospital from January 2021 to December 2022 were selected.After resection of liver cancer tissue and adjacent tissues,liquid nitrogen freezing was performed quickly,and the relative expression of miR-485-5p in tissues and cells was detected by RT-PCR.Hep3B cells from liver cancer were randomly divided into miR-NC group,miR-485-5p group,and miR-485-5p+ML099 group.CCK-8 was used to detect the pro-liferation ability of liver cancer cells,Transwell method was used to detect cell invasion ability,flow cytometry was used to detect cell apoptosis rate,and protein blotting was used to detect the expression of p-MEK,MEK,p-ERK,and ERK proteins in the cells.Results Compared with adjacent tissues(1.18±0.23),the relative expression of miR-485-5p in liver cancer tissue was significantly reduced(0.19±0.14)(P＜0.01).Compared with the normal human liv-er cell line LO2(1.26±0.23),the relative expression of miR-485-5p in human liver cancer cell lines Huh7(0.36±0.09),Hep3B(0.17±0.06),and HepG2(0.31±0.07)was significantly reduced(P＜0.01).Compared with the miR-NC group,the miR-485-5p group showed a significant increase in the relative expression of miR-485-5p(1.25±0.18)and cell apoptosis rate(18.62％±2.11％)in Hep3B cells,while the cell viability(24 h:0.23±0.03,48 h:0.39±0.06,72 h:0.57±0.08),cell invasion number(71.06±8.92),and p-MEK/MEK ratio(0.31±0.04)and p-ERK/ERK ratio(0.42±0.05)were significantly reduced(P＜0.01);Compared with the miR-485-5p group,the miR-485-5p+ML099 group showed a significant decrease in miR-485-5p relative expression(0.31±0.08)and apopto-sis rate(5.18±0.64)in Hep3B cells,while cell viability(24 h:0.24±0.04,48 h:0.61±0.08,72 h:0.98±0.10),number of cell invasions(164.11±17.18),and p-MEK/MEK ratio(0.69±0.08)and p-ERK/ERK ratio(0.89±0.10)were significantly increased(P＜0.01).Conclusion MiR-485-5p shows low expression in liver cancer,and overex-pression of miR-485-5p may inhibit liver cancer cell proliferation and invasion by inhibiting the MEK/ERK signaling pathway,and induce liver cancer cell apoptosis.
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