Objective To investigate the mechanism of m6A methyltransferase METTL3 in colon cancer cells through β-arrestin 1/p65.Methods The expression of METTL3,β-arrestin 1,PCNA,Cyclin D1 and Cyclin E in colon cancer cell line HCT116 were determined by quantitative PCR and Western blot.The mechanism of MET-TL3,β-arrestin 1,p65 in the proliferation of colon cancer cells was studied by cell transfection and cell cloning ex-periments.Results The expression of METTL3 and β-arrestin 1 was increased in colon cancer cells(With actin as the reference gene,compared with the normal control group,P＜0.05).After transfection with shRNA,in cell clon-ing test,the number of clones was 465±42 in the control group and 85±37 in the transfection group(P＜0.05).The ex-pression of cell proliferating protein PCNA,Cyclin D1 and Cyclin E was also significantly decreased(P＜0.01,P＜0.05 and P＜0.01,respectively).In colon cancer cells,we found increased expressions of METTL3,β-arrestin 1 and p-p65(All P＜0.05).Subsequently,the expression of METTL3 was silenced in HCT116 cells,and the expression of β-arrestin 1 and p-p65 in the cells was significantly decreased(All P＜0.05).Conclusion METTL3 affects the prolifera-tion of colon cancer cells and may be involved in the development of cancer through β-arrestin 1/p65 signaling pathway.
关键词
结肠癌/N6-甲基腺苷/甲基转移酶样蛋白3/β-抑制蛋白1)/核因子Kappa/B
Key words
Colon cancer/m6A/Methyltransferase like protein 3(METTL3)/β-arrestin 1/Nucle-ar transcription factor Kappa B(NF-κB)
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