Effect of overexpression of HOXA5 on proliferation of human thyroid cancer cells and its mechanism
Objective:To investigate the effect of homeobox A5(HOXA5)on the biological function of human thyroid cancer TPC1 cells and its possible mechanism.Methods:The expression of HOXA5 mRNA and protein in TPC1 cells was analyzed by RT-qPCR and Western blotting.Negative control plasmid(OE-NC)and HOXA5 overexpression plasmid(OE-HOXA5)were transfected into TPC1 cells using lentiviral transduction and the transfection efficiency was verified.Cell proliferation,colony forming ability and cell cycle were detected by CCK-8,colony formation assay and flow cytometry,respectively.The expression of proliferation related proteins and AKT pathway related proteins were detected by Western blotting.Results:The expression levels of HOXA5 mRNA and protein in thyroid cancer cells were significantly lower than that in thyroid follicular epithelial cells.After up-regulation of HOXA5 expression,the proliferation and clonogenesis of TPC1 cells were significantly inhibited,and the percentage of cells in G0/G1 phase was significantly increased,but the percentage of cells in S phase was decreased.The expression levels of proliferation-related proteins Ki67,c-Myc,as well as key proteins in the AKT signaling pathway,p-GSK3β,and p-AKT were significantly down-regulated.Conclusion:HOXA5 is down-regulated in thyroid cancer,and overexpression of HOXA5 gene can inhibit the proliferation and clonogenesis of thyroid cancer cells,and its mechanism may involve the activation of AKT signaling pathway.
国家科技期刊平台
NSTL
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