Effectiveness of a newly self-made carrier for cryopreservation of mouse embryos
Objective:To investigate the feasibility of cryopreservation of ICR mouse cleaved embryos and blastocysts with our self-made carrier.Methods:The vitrification effect of mouse cleavage embryos and blastocyst were compared between our self-made Cryotip and commercial Cryotop.The data of cleavage embryos recovery rate,blastocyst formation rate,blastocyst recovery rate and hatching rate in the Cryotip group and the Cryotop group were analyzed.Results:There was no significant difference in the cleavage embryos recovery rate,blastocyst formation rate and blastocyst hatching rate of mice cryopreserved in Cryotip and commercial Cryotop groups.The blastocyst hatching rate,recovery rate,and blastocyst formation rate were slightly lower in the Cryotip group compared with the Cryotop group.There was no significant difference in the recovery rate and blastocyst hatching rate in mouse blastocyst cryopreservation.Conclusion:Our self-made carrier Cryotip shows close effect in cryopreservation of ICR mouse cleavage embryos and blastocysts that are comparable to the commercial carrier Cryotop.
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