Influence of Baicalein on blood brain barrier in rats with bacterial meningitis by regulating PI3K/Akt/NF-κB signal pathway
Objective:To investigate the influence of Baicalein(Bai)on blood brain barrier in rats with bacterial meningitis(BM)by regulating phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/nuclear factor-κB(NF-κB)signal pathway.Methods:BM model of rats was prepared.The rats were divided into control group,model group,Bai group(intraperitoneal injection of 30 mg/kg Baicalein),and Bai+inhibitor group(intraperitoneal injection of 30 mg/kg Baicalein+7.5 mg/kg LY294002).The rats were evaluated by Loeffler neurobehavioral score.H-E staining was used to observe the pathological changes in rat brain tissue.Evans blue staining was used to detect the permeability of blood brain barrier.The interleukin-1β(IL-1β),interleukin-6(IL-6)levels and white blood cell count(WBC),water content of brain tissue,aquaporin 4(AQP4),atretic protein-5(claudin-5),and PI3K/Akt/NF-κB pathway related protein expressions in the cerebrospinal fluid(CSF)were detected.Results:The brain tissue structure of the rats in the control group was normal,and the cells were arranged in order.Cells in the brain of the rats in the model group were disorganized,with a large number of inflammatory cells infiltrating.Compared with the control group,the Loeffler score and the expression levels of AQP4,claudin-5,p-PI3K and p-Akt in the brain tissue of the rats in the model group were significantly reduced,the levels of IL-1β,IL-6 and WBC in CSF,the content of EB,brain water content,and the expression level of p-NF-κB p65 were significantly increased.The pathological damage and inflammatory cells in the Bai group were less than those in the model group.Compared with the model group,the Loeffler score and the expression levels of AQP4,claudin-5,p-PI3K and p-Akt in the brain tissue of the rats in the Bai group were significantly increased,the levels of IL-1β,IL-6 and WBC in CSF,the content of EB,brain water content,and the expression level of p-NF-κB p65 were significantly decreased.LY294002 could partly reverse the effect of Baicalein on the permeability of blood brain barrier in BM rats.Conclusion:Baicalein can improve brain edema and blood-brain barrier permeability in BM rats,which may be related to the regulation of PI3K/Akt/NF-κB signaling pathway.
Baicaleinbacterial meningitisphosphatidylinositol 3-kinase/protein kinase B/nuclear factor-κB signal pathwayblood brain barrier
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