Expression of RP11-386G11.10 in triple-negative breast cancer tissues and its effect on cell proliferation and migration
Objective:To explore the expression of lncRNA RP11-386G11.10 in triple-negative breast cancer tissue and its effect on the proliferation and migration of triple-negative breast cancer cells through regulating the miR-1299-3p/GLUT-1 molecular axis.Methods:A total of 46 cases of triple-negative breast cancer tissue and adjacent tissue were collected,and RT-qPCR was used to detect the presence of RP11-386G11.10 in triple-negative breast cancer tissue,the expression of RP11-386G11.10 in normal breast epithelial MCF-10A cells and that in triple-negative breast cancer cell lines(MDA-MB-435,CAL-51,BT-549,MDA-MB-231).BT-549 cells were infected with si-NC lentivirus and si-RP11-386 G11.10 lentivirus,and they were named si-NC group and si-RP11-386G11.10 group respectively.The proliferation and migration abilities of BT-549 cells were detected through colony formation assay and cell scratch assay,respectively.RT-qPCR was used to detect the expression of miR-1299-3p and GLUT-1 mRNA in BT-549 cells after infection.A dual-luciferase reporter gene experiment was used to verify the targeting relationship between RP11-386G11.10 and miR-1299-3p.RT-qPCR was used to detect the expression of GLUT-1 mRNA in triple-negative breast cancer tissues.The Pearson method was used to detect the relationship between the expression of RP11-386G11.10 and GLUT-1 mRNA in triple-negative breast cancer tissues.Western blotting was used to detect the effect of silencing RP11-386G11.10 on the expression of GLUT-1 protein and JAK2/STAT3 pathway proteins in BT-549 cells.Results:Compared with adjacent tissues,the expression of RP11-386G11.10 in triple-negative breast cancer tissues was significantly increased.Compared with MCF-10A cells,the expression levels of RP11-386G11.10 in triple-negative breast cancer cell lines(MDA-MB-435,CAL-51,BT-549,MDA-MB-231)were all significantly increased.Compared with BT-549 cells in the si-NC group,the proliferation and migration abilities of cells in si-RP11-386G11.10 group were significantly reduced.Compared with si-NC group,miR-1299-3p expression was significantly increased and GLUT-1 mRNA expression was significantly decreased in si-RP11-386G11.10 group.RP11-386G11.10 could target and complementally bind to miR-1299-3p.Compared with adjacent tissues,GLUT-1 mRNA expression was significantly increased in triple-negative breast cancer tissues.Pearson analysis showed that the expression levels of RP11-386G11.10 and GLUT-1 mRNA in triple-negative breast cancer tissues were positively correlated.Compared with BT-549 cells in the si-NC group,GLUT-1 protein expression in BT-549 cells in the si-RP11-386G11.10 group was significantly reduced,and the JAK2/STAT3 molecular pathway transduction was inhibited.Conclusion:The expression of RP11-386G11.10 is significantly up-regulated in triple-negative breast cancer.Silencing RP11-386G11.10 can inhibit the proliferation and migration abilities of triple-negative breast cancer BT-549 cells.Its mechanism of action may be related to the targeted regulation of miR-1299-3p/GLUT-1 molecular axis.
triple-negative breast cancerlong non-coding RNARP11-386G11.10microRNAglucose transporter-1
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