Effect of modified Tongqiao decoction on expression of sPD-L1 and Tim-1 in serum of allergic rhinitis model rats
Objective:To study the effects of Tongqiao decoction on the expression of soluble programmed death ligand-1(sPD-L1)and T cell immunoglobulin domain and mucin domain protein 1(Tim-1)in serum of allergic rhinitis(AR)model rats.Methods:After successful modeling,SPF grade SD rats were divided into model group,low-dose Tongqiao decoction group,high-dose Tongqiao decoction group,and dexamethasone group.Behavioral scores of rats in each group were assessed.The levels of IFN-γ and IL-4 were measured using the ELISA method.The pathological morphological structure of the rat nasal mucosa tissue was observed using H-E staining.The expression of sPD-L1 and Tim-1 mRNA and proteins was detected by Real-time PCR and Western blotting.Results:Compared with the healthy group,the model group showed an increase in behavioral scores,IL-4 levels,Tim-1 mRNA and protein expression,and IFN-γ levels,sPD-L1 mRNA and protein expression were all reduced.Compared with the model group,the behavioral scores,IL-4 levels,Tim-1 mRNA and protein expression were all reduced,and the IFN-γ level,sPD-L1 mRNA and protein expression all increased in the low dose and high dose Tongqiao decoction groups,as well as the dexamethasone group;Compared with the low-dose group,the high-dose Tongqiao decoction group and dexamethasone group showed decreased behavioral scores,IL-4 levels,Tim-1 mRNA and protein expression,and increased IFN-γ levels,sPD-L1 mRNA and protein expression.Compared with the high-dose Tongqiao decoction group,the dexamethasone group showed an increase in behavioral scores,IL-4 levels,Tim-1 mRNA and protein expression,and a decrease in IFN-γ levels,sPD-L1 mRNA and protein expression.The model group showed edema in the submucosal layer of the nose,with a large amount of inflammatory cell infiltration.Compared with the model group,the low-dose Tongqiao decoction group,high-dose Tongqiao decoction group,and dexamethasone group all showed improvement.Conclusion:The modified Tongqiao decoction was found to improve the serum sPD-L1 level,reduce the Tim-1 level,and regulate the Th1/Th2 ratio in AR rats.
Tongqiao decoctionallergic rhinitissoluble programmed death ligand-1T cell immunoglobulin domain and mucin domain protein 1rat
万方数据
维普
