首页|外源性胆红素对大鼠脑缺血再灌注损伤的预防作用及其机制

外源性胆红素对大鼠脑缺血再灌注损伤的预防作用及其机制

扫码查看
目的:研究外源性胆红素对脑缺血再灌注损伤(I/R)的作用及其对凋亡的影响.方法:SD大鼠随机分为假手术组、模型组(I/R组)、5 mg/kg胆红素组(I/R+5 mg组)、10 mg/kg胆红素组(I/R+10 mg组)和15 mg/kg胆红素组(I/R+15 mg组),造模前连续给药7 d,采用线栓法建立大鼠脑I/R模型.造模 24 h 后取材,氯化三苯基四氮唑染色测定脑梗死面积,H-E染色观察大鼠脑梗死区域病理改变,TUNEL染色检测大鼠脑组织凋亡细胞,免疫荧光组织化学检测大鼠脑p-caspase 3水平.结果:与I/R组相比,I/R+10 mg组脑梗死面积显著降低;与I/R组相比,I/R+5 mg组和I/R+10 mg组脑组织形态较为正常;与I/R组相比,I/R+10 mg组脑组织TUNEL阳性细胞数量显著减少,I/R+5 mg组、I/R+10 mg组和I/R+15 mg组脑组织p-caspase 3阳性细胞数量显著减少.结论:外源性胆红素在一定剂量范围内对大鼠脑缺血再灌注损伤有预防作用,其保护机制与抑制凋亡有关.
Preventive effect and mechanism of exogenous bilirubin on cerebral ischemia reperfusion injury in rats
Objective:To study the effect of exogenous bilirubin on cerebral ischemia reperfusion injury(I/R)and apoptosis.Methods:SD rats were selected and randomly divided into Sham group(Sham group),modeling group(I/R group),5 mg/kg bilirubin group(I/R+5 mg group),10 mg/kg bilirubin group(I/R+10 mg group),and 15 mg/kg bilirubin group(I/R+15 mg group).The rats were given medicine for seven days before modeling.The plugging filament method was used to establish the rats'cerebral ischemia-reperfusion injury model.24 h after the modeling was completed,specimens were taken for measurement.The infarct volume was determined by triphenyl tetrazolium chloride staining.Pathological changes of cerebral infarction area were observed by H-E staining.TUNEL staining was used to detect the apoptotic cells and immunofluorescence histochemistry was used to detect the positive cells of p-caspase 3 in the brain tissue.Results:Compared with the I/R group,the infarct size of the I/R+10 mg group was significantly reduced.Brain morphology of the I/R+5 mg and I/R+10 mg groups was more normal compared with the modeling group.Compared with the I/R group,the number of TUNEL-positive cells in the brain tissue of the I/R+10 mg group was significantly reduced,and the number of p-caspase 3 positive cells in the brain tissue of I/R+5 mg,I/R+10 mg,and I/R+15 mg groups was significantly reduced.Conclusions:Exogenous bilirubin can prevent cerebral ischemia-reperfusion injury in rats within a certain dose range,and its protective mechanism is related to the inhibition of apoptosis.

brainischemia reperfusion injuryexogenous bilirubinapoptosisrat

李丹、斯依提·阿木提、恰合班·恩特马合、刘艳梅、孟新玲

展开 >

新疆医科大学,附属中医医院,乌鲁木齐 830000

新疆医科大学,基础医学院人体解剖学教研室,乌鲁木齐 830054

缺血再灌注损伤 外源性胆红素 凋亡 大鼠

新疆维吾尔自治区科学技术厅青年基金项目新疆医科大学附属中医医院院级课题

2022D01C178ZYY2020ZD01

2024

解剖学杂志
中国解剖学会

解剖学杂志

CSTPCD
影响因子:0.407
ISSN:1001-1633
年,卷(期):2024.47(3)
  • 6