Objective:To provide a reference for studies related to rat peripheral blood mononuclear cells(PBMNCs),we investigated the composition and age-related changes in PBMNCs isolated from SD rats.Methods:PBMNCs were isolated from male SD rats aged 3,6,and 12 months using lymphocyte separation medium and discontinuous density gradient centrifugation.The types and numbers of cells in PBMNCs,as well as the age-related changes in lymphocyte and monocyte content,were analyzed.Flow cytometry and immunofluorescence were utilized to quantify the number and assess the age-related trends of stem/progenitor cells within PBMNCs.Results:The different cellular components of PBMNCs were clearly separated,and red blood cells and granulocytes were effectively removed.The number of lymphocytes and monocytes in PBMNCs decreased significantly with age.In SD rat PBMNCs,four stemness-related markers(Nanog,OCT4,SOX2,and SSEA4)were expressed.Among them,Nanog exhibited the lowest positive expression,while SSEA4 showed the highest,reaching 23.1%.Overall,the content of PBMNCs stem/progenitor cells in PBMNCs was highest in six-month-old rats.Conclusions:SD rats have a higher content of stem/progenitor cells in their PBMNCs,which suggests that the therapeutic effect of animal experiments might be better than in human applications.For experimental studies involving the transplantation of SD rat PBMNCs for disease treatment,it is recommended to use rats younger than 6 months.
peripheral blood mononuclear cellstemness-related markerage-related changeSD rat
国家科技期刊平台
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