Effect of stachydrine on the malignant biological characteristics of bladder cancer cells by regulating YAP/TAZ signal pathway
Objective:To investigate the influence of stachydrine(STA)on the malignant biological behavior and glycolysis of bladder cancer cells by regulating Yes-associated protein(YAP)/transcriptional coactivator with PDZ-binding motif(TAZ)signaling pathway.Methods:Bladder cancer T24 cells,taken as the research subject,were divided into control group(without drug treatment),low dose(STA-L),medium dose(STA-M),high dose(STA-H)STA groups,YAP/TAZ signaling pathway activator(GA-017)group,and GA-017+STA-H group.Cell proliferation activity was detected by CCK-8 method.Clonogenic assay was used to detect the clonogenic capacity of cells.Transwell assay was used to measure the invasive ability of cells.Cell apoptosis was detected by flow cytometry.The levels of factors affecting glycolysis(ATP,lactic acid production,glucose consumption)were detected with the kits,and the protein expressions of caspase-3,Bcl-2,MMP-9,YAP,and TAZ were detected by Western blotting.Results:Compared with the control group,the proliferation activity,colony formation rate,invasion number,MMP-9 protein expression,Bcl-2 protein expression,ATP level,lactic acid production,and glucose consumption of T24 cells in the three STA groups and the GA-017+STA-H group were decreased obviously,the apoptosis rate and caspase-3 expression were increased obviously.The expression levels of YAP and TAZ proteins in the STA-M,STA-H groups and the GA-017+STA-H group were decreased obviously;and the above indexes in the GA-017 group showed an opposite trend to those in the STA-H group.Compared with the STA-H group,the GA-017+STA-H group could reverse the inhibition of STA on the malignant biology and glycolysis of T24 cells to some extent.Conclusion:STA can inhibit the proliferation,invasion,glycolysis of bladder cancer cell T24,and promote the apoptosis of T24 cells by inhibiting the expression of YAP/TAZ signaling pathway.
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