Cordycepin induces autophagy and apoptosis through regulating Dickkopf-related protein 1/β-catenin signal pathway to inhibit diffuse large B cell lymphoma
Objective:To investigate whether cordycepin could induce autophagy and apoptosis through regulating Dickkopf-related protein 1(Dkk1)/β-catenin signal to inhibit diffuse large B cell lymphoma(DLBCL).Methods:DLBCL SU-DHL-4 cells were selected for research.Cell viability was detected by MTT assay.DLBCL SU-DHL-4 cells were treated with different concentrations of cordycepin and divided into control group,cordycepin 20 µg/mL group,cordycepin 40 µg/mL group,and cordycepin 80 µg/mL group.SU-DHL-4 cells were transfected with si-DDK1 and divided into control group,cordycepin 80 µg/mL group,and cordycepin 80 µg/mL+si-DDK1 group.Cell apoptosis rate was detected by the flow cytometry.The number of microtubule-associated protein light chain 3(LC3)positive cells was detected by fluorescence immunocytochemistry.The protein relative expression levels of cleaved caspase-8,cleaved caspase-3,cleaved caspase-9,cleaved poly-ADP-ribose polymerase(PARP1),becline-1,autophagy-related protein 7(Atg7),LC3Ⅱ,DDK-1 and β-catenin were detected by Western blotting.Results:Compared with the control group,after intervention with 40 and 80 µg/mL cordycepin,the apoptosis rate of SU-DHL-4 cells was increased,and the positive expression of LC3+was risen significantly,and the relative protein expression levels of cleaved caspase-8,cleaved caspase-3,cleaved caspase-9,cleaved PARP1,becline-1,ATG7,LC3Ⅱ/LC3Ⅰ,and DDK-1 were all up-regulated while the relative protein expression of β-catenin was down-regulated.Compared with the cordycepin 80 µg/mL group,the relative protein expression of DDK-1 was down-regulated in the cordycepin 80 µg/mL+si-DDK1 group,while the relative protein expression of β-catenin was up-regulated.At the same time,SU-DHL-4 cell apoptosis rate was reduced,and the positive expression of LC3+was significantly reduced,and the relative protein expression levels of cleaved caspase-8,cleaved caspase-3,cleaved caspase-9,cleaved PARP1,becline-1,ATG7,and LC3Ⅱ/LC3Ⅰ were all down-regulated.Conclusion:Cordycepin can induce autophagy and apoptosis through regulating Dkk1/β-catenin signal to inhibit DLBCL.
diffuse large B cell lymphomacordycepinapoptosisautophagyDickkopf-related protein 1/β-catenin signaling pathway
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