miR-126-5p靶向MAPK1对甲状腺癌顺铂耐药细胞增殖和侵袭的影响
Effect of miR-126-5p targeting MAPK1 on proliferation and invasion of thyroid cancer cisplatin-resistant cells
李璐 1孙弟波 1李萍 1李思宇 1马海燕 1张泽天2
作者信息
- 1. 北方学院附属第一医院血管腺体外科,张家口 075000
- 2. 北方学院附属第一医院消化内科,张家口 075000
- 折叠
摘要
目的:研究miR-126-5p对甲状腺癌细胞顺铂耐药性和耐药细胞增殖侵袭的影响及机制.方法:RT-qPCR检测细胞miR-126-5p 和MAPK1 mRNA表达水平.将细胞分为对照组、miR-126-5p mimic组、miR-NC组、miR-126-5p mimic+pc-MAPK1组和miR-126-5p mimic+pc-NC组.CCK-8法测定细胞顺铂抗性和增殖情况,Transwell测定细胞侵袭,双荧光素酶报告验证靶向关系,免疫印迹检测MAPK1蛋白表达水平.结果:与正常组织和细胞相比,甲状腺癌组织和细胞中 miR-126-5p水平显著下调;与甲状腺癌细胞系TPC-1相比,甲状腺癌顺铂耐药细胞系TPC-1/CDDP中miR-126-5p mRNA表达显著下调.TPC-1细胞、TPC-1/CDDP细胞中,与对照组相比,miR-126-5p mimic组细胞CDDP的IC50值、细胞增殖和每视野侵袭细胞数目均显著降低,miR-126-5p靶向下调MAPK1.过表达MAPK1逆转miR-126-5p过表达对细胞顺铂抗性的IC50值、细胞增殖和每视野侵袭细胞数.结论:miR-126-5p过表达通过靶向下调MAPK1增强TPC-1和TPC-1/CDDP细胞顺铂敏感性,并抑制细胞增殖和侵袭.
Abstract
Objective:To investigate the effect and mechanism of miR-126-5p on cisplatin resistance,proliferation and invasion of thyroid cancer cells.Methods:The expression of miR-126-5p and MAPK1 mRNA in the cells were detected by RT-qPCR.The cells were divided into control group,miR-126-5p mimic group,miR-NC group,miR-126-5p mimic+pc-MAPK1group,miR-126-5p mimic+pc-NC group.The cell cisplatin(CDDP)resistance and proliferation were measured by CCK-8 assay,and invasion was measured by Transwell,the targeting relationship was verified by the dual-luciferase reporter assay,and expression level of MAPK1 protein was detected by Western blotting.Results:Compared with normal tissues and cells,the mRNA level of miR-126-5p was significantly down-regulated in thyroid cancer tissues and cells.Compared with the thyroid cancer cell line TPC-1,the expression of miR-126-5p in thyroid cancer cisplatin-resistant cell line TPC-1/CDDP was significantly down-regulated.Either in TPC-1 cells or TPC-1/CDDP cells,compared with the control group,the IC50 value of CDDP,cell proliferation,and the number of invasive cells per visual field of the miR-126-5p mimic group cells were all significantly reduced.The miR-126-5p targeted the down-regulation of MAPK1.Overexpression of MAPK1 reversed the effect of miR-126-5p overexpression on the IC50 value of cell CDDP,cell proliferation and the number of invasive cells per field.Conclusion:The miR-126-5p overexpression enhances the cisplatin sensitivity of TPC-1 and TPC-1/CDDP cells by targeting the down-regulation of MAPK1,and inhibits cell proliferation and invasion.
关键词
miR-126-5p/MAPK1/甲状腺癌/顺铂/增殖/侵袭Key words
miR-126-5p/MAPK1/thyroid cancer/cisplatin/proliferation/invasion引用本文复制引用
基金项目
2020年度河北省医学科学研究课题计划(20200500)
出版年
2024
国家科技期刊平台
NSTL
万方数据