首页|白纹伊蚊气味受体基因OR21a的克隆及定量分析

白纹伊蚊气味受体基因OR21a的克隆及定量分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
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本文克隆白蚊伊蚊气味受体基因OR21a并分析其是否与搜寻宿主或吸血行为相关.通过设计简并引物,PCR扩增得到白纹伊蚊气味受体基因OR21a片段,采用SYBR荧光定量PCR方法,以白纹伊蚊β-肌动蛋白基因作为内参,比较了OR21a基因在未吸血雌蚊、雄蚊、打断吸血雌蚊和饱血雌蚊头部表达量的差异.结果显示,白纹伊蚊OR21a基因在未吸血雌蚊头部表达量最高,饱血雌蚊头部表达量最低,而在雄蚊头部的表达量介于打断吸血雌蚊和饱血雌蚊之间.本研究发现白蚊伊蚊会依据不同的生理需求改变OR21a基因的表达量,推测可能与白纹伊蚊搜寻宿主及吸血行为有一定的相关性.
CLONING AND EXPRESSION ANALYSIS OF ODORANT RECEPTOR GENE (OR21A) IN AEDES ALBOPICTUS
Sequence of the odorant receptor gene (OR21a) of Aedes albopictus were identified by using degenerate primers.Expression of OR21a mRNA was investigated by quantitative Real-Time RCR in the heads of adult male,interrupt sucking female,engorged and non-feeding adult female.Levels of OR21 a mRNA were normalized to mRNA encoding β-action.The results showed that OR21 a were differentially expressed in the heads of adult male,feeding-interrupted female,engorged and non-feeding adult female.The expression level of non-feeding adult female,feeding-interrupted female,adult male and engorged adult female was reduced successively.The expression level of OR21a changed for need.Maybe the OR21a gene has relationship with the behavior of blood feeding.

Aedes albopictusOR21aReal-time PCRBlood-feeding behavior

阎婷、李海滔、赵明慧、苏建新、董言德、李春晓、赵彤言

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军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071

白纹伊蚊 气味受体基因OR21a 定量PCR 吸血行为

国家科技重大传染病专项课题

2012ZX10004219

2014

10.3969/j.issn.1005-0507.2014.02.006

寄生虫与医学昆虫学报
中国动物学会,中国昆虫学会,军事医学科学院微生物流行病研究所

寄生虫与医学昆虫学报

CSTPCD北大核心
影响因子：0.317
ISSN：1005-0507
年,卷(期)：2014.21(2)
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