Objective To investigate the effect of enhanced Dulbecco's modified Eagle's medium(DMEM)on the cultivation of Blastocystis hominis.Methods A total of 329 clinical samples were selected to compare the growth of B.hominis using enhanced DMEM and the iodine solution direct smear method.The morphology,minimum detection limit and growth curve of B.hominis were compared between the conventional DMEM culture medium and the enhanced DMEM culture method.Results In 329 clinical specimens,the positive rate for B.hominis was 5.17％(17/329)in the enhanced DMEM culture and 1.52％(5/329)in the iodine solution stained smears;the difference was statistically significant(P＜0.01).Conclusion The enhanced DMEM culture method can be used for the proliferation and culture of B.hominis in vitro.
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