SUBCELLULAR LOCALIZATION OF AMINO ACID TRANSPORTER CPAAT1 IN CRYPTOSPORIDIUM PARVUM
Objectives To investigate the subcellular localization of amino acid transporter 1(CpAAT1)in Cryptosporidium parvum.Methods Based on bioinformatics analysis of the amino acid sequence of CpAAT1,specific polypeptide fragments were designed and synthesized for rabbit polyclonal antibodies.The specificity of the antibody was verified using western blotting and the subcellular localization of the protein was analyzed using an indirect immunofluorescence assay(IFA).Results The CpAAT1 polyclonal antibody specifically recognized the native protein of C.parvum and was expressed in all life stages of the parasite.At the sporozoite stage,CpAAT1 was localized on the surface of sporozoites,and at the meront stage,the protein was localized on the surface of the mature merozoite membrane.At the sexual reproduction stage,both male and female gametes can be labeled with the antibody.Conclusions This study provides novel insights that are useful for further exploration of the mechanisms of amino acid transporters in C.parvum.
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