REGULATORY EFFECTS OF MMU-MIR-8114 AND MMU-MIR-3473B IN ALLERGIC MICE INDUCED BY ECHINOCOCCOSIS
Objective This study aimed to explore the regulatory roles of microRNAs(miRNAs)in mice with allergic reactions induced by Echinococcus granulosus.Methods A mouse model of Echinococcosis was established,and spleen mononuclear cells were collected for transcriptomic sequencing.The differential expressions of mRNAs and miRNAs between allergic and non-allergic mice were compared.The target regulatory relationships between mRNAs and miRNAs were predicted using TargetScan and miRanda softwares,and core genes were identified through protein-protein interaction(PPI)network analysis.The biological functions of these core genes were further explored via enrichment analysis.Finally,key results were validated using qRT-PCR and Western blot.Results A total of 1 642 differentially expressed mRNAs were identified between allergic and non-allergic mice.Among the 18 differentially expressed miRNAs,60 target genes were identified,with the top 10 genes having the highest connectivity in the PPI network-deemed as core genes.Enrichment analysis showed that these core genes are primarily involved in the NF-kappa B signaling pathway.Based on the negative regulatory effects between miRNAs and mRNAs and the dual-luciferase reporter system,mmu-miR-8114 was found to target Atf3 and mmu-miR-3473b to target Myd88,both of which are related to the NF-kappa B signaling pathway.qRT-PCR validation showed that mmu-miR-8114 and mmu-miR-3473b were downregulated in allergic mice,while Atf3,Myd88,and Socs3 were upregulated.Increased activity of the NF-kappa B signaling pathway was observed in allergic mice through qRT-PCR and Western blot results.Conclusions This study reveals the significant role of mmu-miR-8114 and mmu-miR-3473b in regulating mouse allergic reactions through the NF-kappa B signaling pathway,offering new insights into the mechanisms behind allergic reactions caused by Echinococcus granulosus infection.
EchinococcosisAllergyMicroRNAsNF-kappa B signaling pathway
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