天津口岸首次截获四斑大蠊(蜚蠊科:大蠊属)
PERIPLANETA QUADRINOTATA,A COCKROACH WAS FIRSTLY INTERCEPTED AT TIANJIN PORT IN CHINA
郭强 1赵丹云 1张琛 1田卉 1祁军 1马毅1
作者信息
- 1. 天津国际旅行卫生保健中心(天津海关口岸门诊部),天津 300457
- 折叠
摘要
目的 对天津口岸首次从泰国入境集装箱中捕获的蜚蠊进行形态描述和DNA测序,为国境口岸蜚蠊鉴定提供参考.方法 2024 年 1 月 5 日,天津口岸从泰国入境集装箱中捕获 1 只雄性蜚蠊,对该蜚蠊进行形态鉴定并描述.取蜚蠊后足 1 条,提取基因组DNA,扩增目的片段并测序.结果 经过形态学鉴定,依据前胸背板两对浅色斑块的典型特征,确定该蜚蠊为四斑大蠊Periplaneta quadrinotata,通过GenBank检索相关序列构建系统进化树,进一步印证该蜚蠊为大蠊属.结论 四斑大蠊在泰国等地有相关报道,在我国未见相关研究及报道,此次截获蜚蠊为全国口岸首次截获.为防止该蜚蠊通过国际贸易的集装箱传入我国,本次研究对四斑大蠊进行形态特征描述及DNA测序,以供口岸检疫人员和昆虫研究人员鉴别参考.
Abstract
Objective Periplaneta quadrinotata was captured by Tianjin Customs at Tianjin Port for the first time from a freight container on January 5,2024.This study aimed to provide a reference including DNA sequence for cockroach species identification at frontier ports.Methods Primary morphological characteristics were described,and genomic DNA was extracted from a cockroach hind leg.DNA barcoding based on the mitochondrial COⅠ gene was provided for reference material.Results The diagnostic feature of the specimens is discernable four spots on the pronotum,accordingly,the intercepted cockroach was identified as P.quadrinotata.In addition,the COⅠ genes of the intercepted cockroach is identical to genus Periplaneta with 97％sequence homology,but different from those sequences of any known Periplaneta species deposited in GenBank.Conclusions P.quadrinotata is known to be reported in Thailand and other regions;however,related research have not been available in Chinese literature.Our re-description of the cockroach had been conducted with the intercepted specimens in a freight container based on its morphological characteristics and DNA barcoding features,which would aid custom technicians in making decisions for the quarantined species.
关键词
四斑大蠊/口岸/形态鉴定/DNA测序Key words
Periplaneta quadrinotata/Port/Morphological identification/DNA sequencing引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据