Expression of phospholipase C Gamma 2 in the mouse model of ulcerative colitis
Objective:To investigate the expression and significance of phospholipase C Gamma 2(PLCG2)in ulcerative colitis(UC)mouse model.Methods:Ten male C57BL/6 wild-type(WT)mice aged 8 to 12 weeks were selected,5 of which were used to isolate different intestinal segments,and the other 5 were selected to isolate intestinal epithelial cells(IECs)and colonic lamina propria lymphocytes(LPLs),and total RNA of each intestinal segment and cell was extracted,respectively.The expression of PLCG2 mRNA was detected by reverse transcription PCR(RT-PCR)and real-time fluorescence quantitative PCR(qRT-PCR).Another 15 WT male mice aged 8 to 12 weeks were randomly divided into 3 groups:control group,acute onset group and convalescent group,with 5 mice in each group.Mice in the acute onset and recovery groups were fed with drinking water containing 2.5%dextran sulfate sodium for 5 days and then fed with conventional drinking water,while those in the control group were fed with conventional drinking water.The mice were treated at different stages of UC development and LPLs were extracted.In addition,15 WT male mice aged 8 to 12 weeks were selected to establish the model in the same way and then IECs were extracted.PLCG2 mRNA expression in LPLs and IECs was detected by RT-PCR and qRT-PCR,respectively.Results:There was no significant difference in the expression of PLCG2 mRNA in the colon of WT mice compared with that in the duodenum,jejunum,ileum and cecum(P>0.05).The expression of PLCG2 mRNA in LPLs of WT mice was significantly higher than that in IECs(P<0.05).Compared with the control group,the relative expression of PLCG2 mRNA in the acute onset group(day 3)and convalescent group(day 9)was significantly decreased in LPLs(P<0.05).There was no significant difference in the relative expression of PLCG2 mRNA in IECs among the control group,the acute onset group(day 5)and the convalescent group(day 9)(P>0.05).Conclusion:The expression of PLCG2 mRNA is down-regulated in the LPLs of UC mice,which may play a role in the occurrence and development of UC.
ulcerative colitisphospholipase C Gamma 2(PLCG2)intestinal epithelial cellslamina propria lymphocytes
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维普
