Preparation of tumor microenvironment-responsive GO-DOX nanocarriers and their application in tumor diagnosis and treatment of mouse transplanted tumor
Objective:To prepare a photothermal drug carrier capable of responding to the tumor environment,enabling controlled drug release within tumor tissues for precise targeting and destruction of tumor cells in vivo.Methods:Chemotherapeutic drug doxorubicin(DOX)was loaded into the surface of graphene oxide(GO)carriers to produce GO-DOX nanoparticles,and the change of DOX fluorescence signal was monitored.First,GO-DOX was respectively incubated with mouse breast cancer 4T1 cells and human gastric epithelial GES-1 cells,and the fluorescence intensity of the two cell types was observed at different time points by confocal microscope.Then,the tumor xenograft model of 4T1 mouse was established and the changes of tumor volume of mice were observed.Last,tumor tissues were collected to prepare pathological sections,and the inhibitory effect of GO-DOX nanoparticles combined with near infrared light on xenografts in mice was evaluated.Results:GO-DOX nanoparticles not only had the environmental responsiveness to control drug release,but also were used to fluorescently image tumor cells.The combination of GO-DOX nanoparticles and near-infrared light effectively inhibited tumor growth and reduced the toxic and side effects of DOX on human body.Conclusion:Environment-responsive GO-DOX nanoparticles were successfully prepared,and can be used to effectively inhibite the growth of tumor and realize real-time imaging of tumor cells.
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