Protective effect of Wenyang Jieyu granule on HT22 cells of the hippocampal neurons in mice damaged by corticosterone
Objective:To explore the antidepressant effect and underlying mechanism of Wenyang Jieyu granule(WYJY)by constructing a corticosterone(CORT)injury-induced depression model in mouse hippocampal neurons(HT22)in vitro.Methods:HT22 cells were divided into normal group,model group,WYJY group,and fluoxetine group.Except for the normal group,HT22 cells in the other groups were treated with CORT.WYJY group and fluoxetine group were treated with corresponding drug-containing serum for 24 hours.The HT22 cells in model group added 10%blank serum for 24 hours.CCK-8 method was used to detect cell survival rate.Cell morphology was observed under a microscope.Enzyme linked immunosorbent assay was used to detect LDH release.Hoechst staining was used to observe the survival number of cells.Immunofluorescence assay was used to detect the fluorescence expression of Brdu and Neun and observe the regeneration of HT22 cells.Flow cytometry was used to detect the apoptosis rate of cells.Results:Compared with the normal group,HT22 cells in the model group were severely damaged,the number of cells decreased,and the survival rate was significantly decreased(P<0.01),LDH release was significantly increased(P<0.01),the fluorescence intensity of Brdu/Neun double staining decreased significantly(P<0.01),and the apoptosis rate increased significantly(P<0.01).Compared with the model group,the survival rate of HT22 cells was increased after the effect of serum containing WYJY on CORT injury(P<0.05),the LDH release and apoptosis rate were decreased(P<0.05,P<0.01),the fluorescence intensity of Brdu/Neun and the number of positive cells were significantly increased(P<0.05,P<0.01).Conclusion:WYJY may play an antidepressant role by repairing damaged hippocampal neuron cells,regulating nerve plasticity,inhibiting apoptosis of hippocampal neuron cells and differentiating into new neurons.
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维普
