Intervention effect of blueberry derived exosome-like nanoparticles on MNNG-induced malignant transformation of human gastric epithelial cells
Objective:Blueberry derived exosome-like nanoparticles(BELNs)were extracted and identified,and the intervention effect of BELNs on the malignant transformation of human normal gastric mucosal epithelial cells induced by(N-methyl-N'-nitro-N-nitrosoguallidine,MNNG)was investigated.Methods:The BELNs were extracted by ultracentrifugation,and the BELNs were identified with particle size,Zeta potential and morphology by nano-particle tracking analyzer and transmission electron microscopy.Laser confocal microscopy was used to observe whether BELNs could be endocytosed by transformed gastric mucosal epithelial cells(TGES-1).TGES-1 cells were treated with 200 and 400 μg/mL BELNs for 72 h,then Western blotting was used to analyze the expression of stemness genes,epithelial-mesenchymal transition(EMT)and proliferation related proteins.Results:The results of nano-particle tracking analyzer and transmission electron microscopy showed that BELNs was matched with the characteristics of exosome-like nanoparticles.The results of laser confocal microscopy confirmed that BELNs could be endocytosed by TGES-1 cells.Western blotting showed that 400 μg/mL BELNs inhibited the expression of N-cadherin,E-cadherin,Vimentin,Nanog and proliferating cell nuclear antigen(PCNA)and enhanced expression of E-cadherin in TGES-1 cells.Conclusion:BELNs exert a significant intervention effect on human normal gastric mucosal epithelial cells induced by MNNG.
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