Research on High-speed Data Processing of Time-division Multiplexing Two-photon Imaging System
In order to satisfy the needs of signal demultiplexing,line-field synchronization and pixel value reconstruction in the two-photon microscopy imaging system with high-frequency pulse time-division multiplexed and dual-independent scanning detection,the acquisition and processing of high-speed data are studied;Based on Lab VIEW FPGA,combined with finite state machine,FIFO cache and parallel pipeline technologies,a multifunctional acquisition and processing system is designed,which successfully achieves the lossless demultiplexing of high-speed data and subsequent imaging data processing through the precise timing control,it can cor-rect the image distortion caused by the nonlinear motion of the resonant galvanometer;After debugging,simulation and experimental testing,with single-channel sampling rates of 800 MSPS and 200 MHz FPGA clock,the system can perform complex processing on the corresponding fluorescence signals from different locations generated by 160 MHz time-staggered laser pulses.Practical applica-tions show that the system meets the needs of real-time imaging and provides useful references for researchers in related fields.
time division multiplexingtwo-photon microscopy imaging systemhigh-speed datademultiplexingpixel value re-constructionimage distortionLab VIEW FPGA
万方数据
维普
