The strain LH of goose parvovirus ( GPV) was isolated from the infected young goose in Liuhe District of Nanjing. The virus were propagated in goose embryos of 12-day-old, and showed typical shape of parvovirus under electronic microscope. The goose of 8-day-old were artificially inoculated with the virus sample, infection rate and mortality was 100% , and the clinic symptom and pathological lesion were similar to the natural infected cases of GPV. The GPV antigen from allantoic fluid of infected embryos could react with positive serum of GPV by agar gel precipitation. The strain LH liquid was detected by PCR with the specific primers aim at GPV VP3, and the result showed a bound of 1. 6 kb, coincide with expected bound. Sequence analysis showed 99% -100% nucleotide homology between the amplified product and the other published GPV strains, which indicated the isolated strain LH was goose parvovirus.
NETL
NSTL
维普
万方数据
