首页|南美白对虾冷藏过程中的细菌分离、初步鉴定及菌相分析

南美白对虾冷藏过程中的细菌分离、初步鉴定及菌相分析

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为了明确南美白对虾在冷藏过程中细菌的组成,为开发新的保鲜剂提供指导,利用生理生化检验和16S rDNA序列分析对分离纯化的菌株进行了鉴定,并结合生理生化检验结果和系统进化树进行了分析.结果表明:南美白对虾冷藏期间有8种典型菌株,它们在货架期结束时所占的比例分别为腐败希瓦氏菌36%、嗜水气单胞菌26%、恶臭假单胞菌15%、蜡样芽孢杆菌7%、嗜冷杆菌10%、不动杆菌2%、金黄色葡萄球菌2%、微小杆菌1%,其中腐败希瓦氏菌为南美白对虾冷藏后期的主要优势腐败菌.
Isolation and identification of bacteria in Penaeus vannammei during cold storage and analysis of composition of microorganism
To identify the bacterial composition during cold storage of Penaeus vannammei and providing guidance for the new natural preservative, identification of microorganism strain was performed by using the physiological and biological test, and 16S rDNA sequence analysis. Combined with physiological and biochemical test results and phylogenetic tree analysis , the result demonstrated that there were eight typical microorganism strains in Penaeus vannammei under cold storage. The eight microorganism strains ( and the proportion in the end of shelf life, respectively) were as follows : Shewanella putrefaciens ( 36% ) , Aeromonas hydrophila ( 26% ) , Pseudomonas putida ( 15% ) , Bacillus cereus ( 7% ) , Psychrobacter sp. ( 10% ) , Acinetobacter (2% ) , Staphyloccocus aureus( 2% ) , Microbacterium ( 1% ) . Slzewanella putrefaciens could be considered as dominated strains during cold storage of Penaeus vannammei

赵海鹏、谢晶、严文蓉

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上海海洋大学食品学院,上海,201306

南美白对虾 腐败菌 16SrDNA 保鲜

2010年国家农业成果转化基金上海市优秀学科带头人计划项目上海市教育委员会重点学科建设项目

2010GB2C00014809XD1402000J50704

2011

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCDCSCD北大核心
影响因子:1.093
ISSN:1000-4440
年,卷(期):2011.27(1)
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