Identification of salt tolerant germplasms and screening of related molecular markers in waxy maize at germination and seedling stages
Salt stress affects the growth and development of waxy maize. The aim of the present study was to provide germplasms and molecular information of waxy maize for salt tolerance. For this purpose, a panel consisting of 85 waxy maize inbred lines was measured for five salt tolerance traits, percentage of plant survival, plant survival days, relative germination rate, relative plumule length, and relative radicle axis length, at germination and seedling stage. In addition, the pane) was genotyped by 71 pairs of genome-wide simple sequence repeat (SSR) markers. Linkage disequilibrium of pair-wise loci and population structure were analyzed, and the association analysis between SSR loci and the five measured traits was performed using TASSEL GLM (general linear model) program. The results were as follows. The five salt tolerance traits demonstrated significant difference and wide variation range in waxy maize inbred lines. Five elite inbred lines with better performance under salt stress were selected, among which, NA18 and NA32-2 were the best 340 specific DNA bands were detected in the panel, averagely 4.78 polymorphic fragments for each primer. The value of polymorphism information content (PIC) for each SSR locus varied from 0.249 to 0.876 with an average of 0. 479. A model-based population structure analysis divided the population into five subpopulations, three of which have been reported previously, while the other two are newly developed. A total of nine loci, located on chromosomes 1,2,5,5 and 9, were detected to be associated with the five salt tolerance traits significantly, respectively, which explained phenotypic variation ranging from 7% to 29%. The marker umc2012 on chromosome 1 was detected to be associated with the two traits, percentage of plant survival and plant survival days, simultaneously. These results suggest that the identified germ-plasms and molecular markers in the present study may be useful for the breeding of waxy maize.
NETL
NSTL
万方数据
维普
