Partial sequences of NFAT5 cDNA in two duck breeds, Gaoyou duck and Jinding duck were cloned, and the mRNA expression levels in the pectorale and leg muscles were detected by quantitative RT-PCR at 13-, 17-, 21-, 25-, 27-day embryonic age and at seven days of age. The results showed that the NFAT5 mRNA expressions in the pectorale and leg muscles of Gaoyou duck and Jinding duck were similar, with the highest value at 13 embryonic age. At severn day, the NFAT5 mRNA expression of 7-year-old ducks was significantly higher than that of ducks at 27-day embryonic age ( P<0. 05 ) . The expression levels of NFAT5 in the pectorals and legs of two duck breeds were negatively correlated with the pectoral weights, leg weights, and body weights, while positively correlated with IGF-I mRNA expression ( P<0. 01 ) . These results suggested that there was a synergism between NFAT and IGF-I which may be involved in the regulation of growth and development of duck skeletal muscle.
NETL
NSTL
万方数据
维普
