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红蓝光比例对弱光下黄瓜幼苗光合功能的影响

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为了研究不同比例的红蓝光对弱光下黄瓜叶绿体超微结构和相关光合特性的影响,采用发光二极管( LED)调制不同红蓝光比例,以白光为对照,研究红光,蓝光,红光、蓝光配比7:1和红光、蓝光配比1:1对黄瓜功能叶的影响。结果显示,红光处理下类囊体膜的垛叠严重受挤压,基粒、基质片层毫无规则;最大光化学效率( Fv/Fm )、净光合速率( Pn )、气孔导度( Gs )最低。蓝光处理下叶绿素相对含量( SPAD值)和不平衡系数均最低。红光、蓝光配比1:1处理下的比叶质量、Pn、Gs和光合性能指数( PIcsm )均最高。红光、蓝光配比7:1和白光处理的Pn均显著大于蓝光,但Gs三者间无显著差异。进一步分析表明,弱光下,单纯红光导致黄瓜叶片光合功能失调,蓝光为光合机构高效运行所必须;红光、蓝光配比1:1最有利于黄瓜幼苗叶绿体的发育和高光合速率的实现。
Photosynthesis of Cucumis sativus under weak light in response to different proportions of red to blue light
White 1ight ( contro1) , red 1ight, b1ue 1ight, red to b1ue at 7 : 1 and red to b1ue at 1 : 1 provided by 1ight-emitting diodes were app1ied to study the inf1uences of different combinations of red and b1ue 1ight on cucumber( Cucu-mis sativus) functiona1 1eaves under weak 1ight environment. Resu1ts showed that the stack of thy1akoid membranes of grown 1eaves under red 1ight was severe1y squeezed, and grana and stroma 1ame11ae were irregu1ar. The photochemica1 efficiency ( Fv/Fm ) , net photosynthetic rate ( Pn ) and stomata1 conductance ( Gs ) were the 1owest under red 1ight. Under b1ue 1ight, the SPAD va1ue and the deviation of excitation energy from fu11 ba1ance between PS1 and PS2 (β/α-1 ) were the 1owest. The specific 1eaf weight ( SLW) , PN , Gs , photosynthetic performance index ( PIcsm ) were the 1argest in red to b1ue 1ight at 1 : 1 treatment. A1though Pn in white 1ight treatment and red to b1ue 1ight at 7 : 1 treatment were significant1y greater than that under b1ue 1ight, there was no significant difference in Gs . Cucumber 1eaves grown under red 1ight a1one exhibited dys-functiona1 photosynthetic operation, and b1ue 1ight was indespensab1e for the efficient operation of cucumber photosynthetic apparatus under weak 1ight. Red to b1ue 1ight at 1 : 1, was favorab1e for the deve1opment of ch1orop1ast and high photosyn-thetic rate under weak 1ight.

1ight-emitting diodered and b1ue 1ight ratioCucumis sativusch1orop1ast u1trastructurephotosynthetic characteristic

王晓艳、张晓楠、成后德、崔瑾、许晓明

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南京农业大学生命科学学院,江苏 南京 210095

LED 红蓝光比例 黄瓜 叶绿体超微结构 光合特性

江苏省自然科学基金

Q201024

2014

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCDCSCD北大核心
影响因子:1.093
ISSN:1000-4440
年,卷(期):2014.(6)
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