Analyses of expression patterns of transcription factor MYB10 and anthocyanin synthesis genes in two red skin pear varieties
The objective of this study is to reveal the difference of the coloration mechanism between two red skin pear varieties by analyzing the expression of anthocyanin synthesis related genes.Expression of the genes encoding seven enzymes (PAL,CHS,CHI,F3H,DFR,ANS,F3GT) and transcription factor MYB10 gene were examined by real-time PCR for fruit skin of two pear varieties,Starkrimson and Mantianhong,at three fruit development stages.In Starkrimson,the expression of the anthocyanin synthesis related genes except for PAL increased and then decreased,in agreement with anthocyanin content changes,during the whole stage of fruit development.However,in Mantianhong,the expressions of PAL and F3GT were the highest at fruit color turning and other genes decreased gradually along with fruit development.MYB10 gene were lowly expressed during the whole fruit development in Starkrimson,and reached the highest at fruit color turning in Mantianhong,50 times higher than in Starkrimson.The coloration degree of Starkrimson could be controlled through the coordinated expression of the structural genes.MYB10 might control the coloration in Mantianhong through regulating the expression of key gene F3GT.
red pearanthocyaninexpressionMYB10quantitative real-time PCR
NETL
NSTL
万方数据
维普
