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棉花耐盐相关序列扩增多态性(SRAP)分子标记筛选

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利用相关序列扩增多态性( SRAP)和群体分离分析法( BSA)对棉花耐盐材料×敏盐材料组合的F2群体及26个耐、敏品种(系)进行分析和鉴定,筛选与棉花耐盐相关的分子标记。在88对正反引物组合中,筛选出在2个亲本间具有多态性的引物6对,经F2群体分析,发现1对引物能够在耐盐单株中扩增出350 bp的特异片段,而在敏盐单株中没有。品种鉴定显示,耐盐材料均能扩增出该片段,而敏盐材料没有该片段扩增。推测该片段是与棉花耐盐性紧密连锁的分子标记。
Selection of sequence-related amplified polymorphism molecular marker associated with salt tolerance of cotton
Twenty-six salt tolerant and sensitive materials, and a F2 population derived from the cross between a salt tolerant material and a salt sensitive material were analyzed by sequence-related amplified polymorphism ( SRAP ) marker and bulked segrgant analysis( BSA) method to screen markers associated with salt tolerance of cotton. Six pairs of 88 prim-er combinations were detected polymorphic in both parents, among which, one pair of primer amplified a 350-bp fragment in the tolerant individuals of F2 population, but no band in the susceptible individuals. The fragment were identified exclu-sively in 22 salt tolerant varieties. The results suggested that this marker might be closely linked to salt tolerance in cotton.

cottonsalt tolerancesequence-related amplified polymorphism( SRAP)molecular marker

刘雅辉、王秀萍、鲁雪林、张国新、李强

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河北省农林科学院滨海农业研究所,河北 曹妃甸 063200

棉花 耐盐性 相关序列扩增多态性(SRAP) 分子标记

河北省自然科学基金资助项目

C2014301038

2015

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCDCSCD北大核心
影响因子:1.093
ISSN:1000-4440
年,卷(期):2015.(3)
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